Host: E. coli • AA: 22-292 (MR1), 21-119 (β2-microglobulin) • MW: 32 (MR1) and 12 (β2-microglobulin) kDa
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Measurement and Detection Target(s)
34257MR1 Monoclonal Antibody (Clone 2H7)
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MR1/β2-Microglobulin Complex (human, recombinant)

Item No. 30585

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Class I Histocompatibility Antigen-like Protein
  • Major Histocompatibility Complex Class I-related Gene Protein
  • MHC Class I-related Gene Protein
  • β2-Microprotein
  • β2M
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human MR1 and β2-microglobulin expressed in E. coli
Amino Acids
22-292 (MR1), 21-119 (β2-microglobulin)
MW
32 (MR1) and 12 (β2-microglobulin) kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride
UniProt Accession №
Q95460 (MR1), P61769 (β2-microglobulin)
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    20 µg

    $616

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    Product Description

    MHC class I-related gene protein (MR1) is a non-polymorphic MHC class Ib antigen-presenting cell surface molecule that is required for T cell receptor-mediated activation of mucosal-associated invariant T (MAIT) cells.1,2 MR1 is composed of α1 and α2 domains, which form an antigen-binding pocket, and an α3 domain that interacts with β2-microglobulin, the light chain component of MHC class I molecules that facilitates complex transport to the cell surface and antigen presentation to cytotoxic T cells.2,3 Upon binding of a microbial antigen, MR1 undergoes a conformational change in the endoplasmic reticulum and translocates to the cell surface with β2-microglobulin where it induces MAIT cell activation via an interaction with the MAIT cell T cell receptor and activates various immunomodulatory effects, including cytokine release, initiation of adaptive immune responses, and promotion of tissue repair.4,5 The antigen-binding domain of MR1 binds to vitamin B metabolites, including metabolites of vitamin B2, also known as riboflavin, and vitamin B9 (folic acid; Item No. 20515).6 MHC class I-associated β2-microglobulin exhibits equilibrium exchange with circulating soluble β2-microglobulin and serum levels of β2-microglobulin are increased in patients with cancer, renal failure, systemic amyloidosis, or various autoimmune diseases, including multiple sclerosis.3,7,8,9

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lamichhane, R., and Ussher, J.E. Expression and trafficking of MR1. Immunology 151(3), 270-279 (2017).

    2. Krovi, S.H., and Gapin, L. Structure and function of the non-classical major histocompatibility complex molecule MR1. Immunogenetics 68(8), 549-559 (2016).

    3. Nomura, T., Huang, W.-C., Zhau, H.E., et al. β2-Microglobulin-mediated signaling as a target for cancer therapy. Anticancer Agents Med. Chem. 14(3), 343-352 (2014).

    4. McWilliam, H.E.G., Eckle, S.B.G., Theodossis, A., et al. The intracellular pathway for the presentation of vitamin B-related antigens by the antigen-presenting molecule MR1. Nat. Immunol. 17(5), 531-537 (2016).

    5. McWilliam, H.E.G., and Salio, M. Understanding and modulating the MR1 metabolite antigen presentation pathway. Mol. Immunol. 129, 121-126 (2021).

    6. Kjer-Nielsen, L., Patel, O., Corbett, A.J., et al. MR1 presents microbial vitamin B metabolites to MAIT cells. Nature 491(7426), 717-723 (2012).

    7. Eichner, T., and Radford, S.E. Understanding the complex mechanisms of β2-microglobulin amyloid assembly. FEBS J. 278(20), 3868-3883 (2011).

    8. Stoppini, M., and Bellotti, V. Systemic amyloidosis: Lessons from β2-microglobulin. The Journal of Biological Chemisty 290(16), 9951-9958 (2015).

    9. Bagnato, F., Durastanti, V., Finamore, L., et al. Beta-2 microglobulin and neopterin as markers of disease activity in multiple sclerosis. Neurol. Sci. 24(Suppl 5), S301-S304 (2003).