Host: HEK293 cells • AA: 1-390 (full length) • Tag: C-terminal His • MW: 42.4 kDa for LAP-bound TGF-β1
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Latent TGF-β1 (human, recombinant)

Item No. 30606

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CED
  • DPD1
  • TGFB
Purity
≥95% estimated by SDS-PAGE
Source
Recombinant human C-terminal His-tagged TGF-β1 expressed in HEK293 cells
Amino Acids
1-390 (full length)
MW
42.4 kDa for LAP-bound TGF-β1
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $361

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    500 µg

    $2,345

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    Cayman Chemical
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    Product Description

    Transforming growth factor β1 (TGF-β1) is a multifunctional cytokine and member of the TGF-β superfamily.1,2 It is an extracellular dimeric protein that is produced by multiple cell types, including macrophages, platelets, neutrophils, regulatory T cells, and renal tubular cells, and is the most abundantly expressed isoform of TGF-β. TGF-β1 is expressed as a proprotein that is cleaved in the Golgi to form a latency-associated peptide (LAP) and mature TGF-β1.2 LAP and TGF-β1 remain non-covalently bound until mature TGF-β1 is released from latency by various proteins, including matrix metalloproteinases and plasmin, to regulate immune function, epithelial-mesenchymal transition, and cell proliferation. Severe acute respiratory syndrome coronavirus (SARS-CoV) papain-like protease induces TGF-β1-dependent type I collagen production, a marker of pulmonary fibrosis, in A549 cells and mouse lung.3 Expression of constitutively active TGF-β1 induces Alzheimer’s disease-like cerebrovascular stiffness and vascular hypertrophy in mice.4 Serum and urine levels of TGF-β1 are increased in patients with type 2 diabetes.5 Cayman's TGF-β1 (human, recombinant) protein can be used for ELISA, Western blot, and cell-based assay applications. The LAP-bound form of this protein consists of 370 amino acids and has a calculated molecular weight of 42.4 kDa. By SDS-PAGE, under reducing conditions, the apparent moleular mass of the protein is 16, 38, and 55 kDa for mature TGF-β1, LAP, and LAP-bound TGF-β1, respectively, due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Martelossi Cebinelli, G.C., Paiva Trugilo, K., Badaró Garcia, S., et al. TGF-β1 functional polymorphisms: A review. Eur. Cytokine Netw. 27(4), 81-89 (2016).

    2. Khalil, N. TGF-β: From latent to active. Microbes Infect. 1(15), 1255-1263 (1999).

    3. Wang, C.-Y., Lu, C.-Y., Li, S.-W., et al. SARS coronavirus papain-like protease up-regulates the collagen expression through non-Samd TGF-β1 signaling. Virus Res. 235, 55-66 (2017).

    4. Zhang, X., Huang, W.-J., and Chen, W.-W. TGF-β1 factor in the cerebrovascular diseases of Alzheimer’s disease. Eur. Rev. Med. Pharmacol. Sci. 20(24), 5178-5185 (2016).

    5. Qiao, Y.-C., Chen, Y.-L., Pan, Y.-H., et al. Changes of transforming growth factor beta 1 in patients with type 2 diabetes and diabetic nephropathy: A PRISMA-compliant systematic review and meta-analysis. Medicine (Baltimore) 96(15), e6583 (2017).