A fluorescent probe
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Resorufin (sodium salt)

Item No. 30789

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-hydroxy-3H-phenoxazin-3-one, monosodium salt
CAS Number
34994-50-8
Molecular Formula
C12H6NO3 • Na
Formula Weight
235.2
Purity
≥98%
Emission
580 nm
Excitation
570 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlEthanol: slightly solublePBS (pH 7.2): 10 mg/ml
λmax
225, 479, 540, 578 nm
SMILES
O=C1C=C2OC3=CC([O-])=CC=C3N=C2C=C1.[Na+]
InChi Code
InChI=1S/C12H7NO3.Na/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9;/h1-6,14H;/q;+1/p-1
InChi Key
TYXBZZBYMSVFPR-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

    $54

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    250 mg

    $99

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    500 mg

    $172

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    1 g

    $315

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    Product Description

    Resorufin is a fluorescent probe and the reduced form of the redox indicator dye resazurin (Item No. 14322).1 It can be further reduced to hydroresorufin, which is non-fluorescent. Resorufin has been used in the synthesis of resorufin-based fluorescent probes.2,3 It has also been used for the direct measurement of solvent-solute hydrogen bond dynamics with distinct absorption and emission peaks in solvents with strong hydrogen bonds and overlapping peaks in solvents with weak hydrogen bonds.4 It displays excitation/emission maxima of 570/580 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. O'Brien, J., Wilson, I., Orton, T., et al. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267(17), 5421-5426 (2000).

    2. Mayer, R.T., Jermyn, J.W., Burke, M.D., et al. Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases. Pestic. Biochem. Physiol. 7(4), 349-354 (1977).

    3. Yoon, J.W., Kim, S., Yoon, Y., et al. A resorufin-based fluorescent turn-on probe responsive to nitroreductase activity and its application to bacterial detection. Dyes Pigm. 171, 107779 (2019).

    4. Yu, J., and Berg, M. Resorufin as a probe for the dynamics of solvation by hydrogen bonding. Chem. Phys. Lett. 208(3-4), 315-320 (1993).