Glucose-6-phosphate Isomerase Monoclonal Antibody (Clone 5B6)

Glucose-6-phosphate isomerase (GPI) is a glycolytic enzyme that catalyzes the conversion of D-glucose-6-phosphate (Item No. 20376) to D-fructose-6-phosphate.^1,2 It exists as a dimer where each monomer is composed of a large and small globular domain, which form a cleft that contains the catalytic active site, and a C-terminal tail.^2,3 GPI is ubiquitously expressed and localized to the cytoplasm.^1,2 It also functions as a neurotrophic growth factor and has a role in immunoglobulin synthesis.² GPI is an autoantigen in rheumatoid arthritis (RA).⁴ Immunization with recombinant human GPI induces inflammatory cell infiltration, cartilage destruction, and bone erosion in the inflamed joints of mice, an effect that is reduced in Padi4 knockout mice, which lack peptidyl arginine deiminase 4 (PAD4), an enzyme involved in protein citrullination.⁵ Cayman's Glucose-6-phosphate Isomerase Monoclonal Antibody (Clone 5B6) can be used for ELISA, flow cytometry (FC), and Western blot (WB) applications. The antibody recognizes glucose-6-phosphate isomerase at 63 kDa from human samples.

WARNING This product is not for human or veterinary use.

1. Schaller, M., Burton, D.R., and Ditzel, H.J. Autoantibodies to GPI in rheumatoid arthritis: Linkage between an animal model and human disease. Nat. Immunol. 2(8), 746-753 (2001).

2. Sun, Y.-J., Chou, C.-C., Chen, W.-S., et al. The crystal structure of a multifunctional protein: Phosphoglucose isomerase/autocrine motility factor/neuroleukin. Proc. Natl. Acad. Sci. USA 96(10), 5412-5417 (1999).

3. Cordeiro, A.T., Michels, P.A.M., Delboni, L.F., et al. The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicana reveals novel active site features. Eur. J. Biochem. 271(13), 2765-2772 (2004).

4. Umeda, N., Matsumoto, I., Ito, I., et al. Anti-citrullinated glucose-6-phosphate isomerase peptide antibodies in patients with rheumatoid arthritis are associated with HLA-DRB1 shared epitope alleles and disease activity. Clin. Exp. Immunol. 172(1), 44-53 (2013).

5. Seri, Y., Shoda, H., Suzuki, A., et al. Peptidylarginine deiminase type 4 deficiency reduced arthritis severity in a glucose-6-phosphate isomerase-induced arthritis model. Sci. Rep. 5, 13041 (2015).