Host: E. coli • AA: 1-434 (full length) • Tag: C-terminal His • MW: 66 kDa
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Citrullinated Glucose-6-phosphate Isomerase (human, recombinant)

Item No. 30967

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GPI
  • PGI
  • PHI
  • Phosphoglucose Isomerase
  • Phosphohexose Isomerase
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant C-terminal His-tagged protein expressed in E. coli citrullinated by PAD2
Amino Acids
1-434 (full length)
MW
66 kDa
Formulation (Request formulation change)
25 mM Tris, pH 7.0, with 100 mM sodium chloride, and 10% glycerol
UniProt Accession №
P06744
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $602

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    Product Description

    Glucose-6-phosphate isomerase (GPI) is a glycolytic enzyme that catalyzes the conversion of D-glucose-6-phosphate (Item No. 20376) to D-fructose-6-phosphate.1,2 It exists as a dimer where each monomer is composed of a large and small globular domain, which form a cleft that contains the catalytic active site, and a C-terminal tail.2,3 GPI is ubiquitously expressed and localized to the cytoplasm.1,2 It also functions as a neurotrophic growth factor and has a role in immunoglobulin synthesis.2 GPI is an autoantigen in rheumatoid arthritis (RA).4 Immunization with recombinant human GPI induces inflammatory cell infiltration, cartilage destruction, and bone erosion in the inflamed joints of mice, an effect that is reduced in Padi4 knockout mice, which lack peptidyl arginine deiminase 4 (PAD4), an enzyme involved in protein citrullination.5 Citrullinated GPI autoantibodies have been found in the serum of patients RA.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schaller, M., Burton, D.R., and Ditzel, H.J. Autoantibodies to GPI in rheumatoid arthritis: Linkage between an animal model and human disease. Nat. Immunol. 2(8), 746-753 (2001).

    2. Sun, Y.-J., Chou, C.-C., Chen, W.-S., et al. The crystal structure of a multifunctional protein: Phosphoglucose isomerase/autocrine motility factor/neuroleukin. Proc. Natl. Acad. Sci. USA 96(10), 5412-5417 (1999).

    3. Cordeiro, A.T., Michels, P.A.M., Delboni, L.F., et al. The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicana reveals novel active site features. Eur. J. Biochem. 271(13), 2765-2772 (2004).

    4. Umeda, N., Matsumoto, I., Ito, I., et al. Anti-citrullinated glucose-6-phosphate isomerase peptide antibodies in patients with rheumatoid arthritis are associated with HLA-DRB1 shared epitope alleles and disease activity. Clin. Exp. Immunol. 172(1), 44-53 (2013).

    5. Seri, Y., Shoda, H., Suzuki, A., et al. Peptidylarginine deiminase type 4 deficiency reduced arthritis severity in a glucose-6-phosphate isomerase-induced arthritis model. Sci. Rep. 5, 13041 (2015).