For immunochemical detection of β2-microglobulin
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β2-Microglobulin (dog) Monoclonal Antibody (Clone 5B10)

Item No. 31017

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • B2M
  • IMD43
  • β2-Microglycoprotein
Immunogen
Recombinant dog β2-microglobulin protein (amino acids 28-125)
Clone Designation
5B10
MW
~14 kDa
Formulation (Request formulation change)
100 µg of protein G-affinity purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol, and 0.02% sodium azide
Host
Mouse
Isotype
IgG2a
Applications
FC, IP, WB
Cross Reactivity
(+) β2-Microglobulin
Species Reactivity
(+) Dog(+) Human
UniProt Accession №
E2RN10
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $347

    Add
    Cayman Chemical
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    Product Description

    β2-Microglobulin is a 98-amino acid protein and the light chain component of major histocompatibility complex (MHC) class I molecules.1,2 It is non-covalently associated with the MHC class I α chain and comprises a protein-building subunit of the MHC class I molecule to facilitate complex transport to the cell surface and antigen presentation to cytotoxic T cells. β2-Microglobulin is found in all nucleated cells, as well as in extracellular fluids, including urine and serum, and MHC class I-associated β2-microglobulin exhibits dissociation and equilibrium exchange with circulating soluble β2-microglobulin.1,3 Germline mutations at the β2-microglobulin exon 1/intron 1 splice site have been found in dog mammary gland simple and complex carcinoma tumor samples.4 Cayman's β2-Microglobulin (dog) Monoclonal Antibody (Clone 5B10) can be used for flow cytometry (FC), immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes β2-microglobulin at approximately 14 kDa from dog samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nomura, T., Huang, W.-C., Zhau, H.E., et al. β2-Microglobulin-mediated signaling as a target for cancer therapy. Anticancer Agents Med. Chem. 14(3), 343-352 (2014).

    2. Nakajima, Y., Hoshi, F., Higuchi, S., et al. The complete amino acid sequence of dog β2-microglobulin. J. Vet. Med. Sci. 61(5), 517-521 (1999).

    3. Nakajima, Y., Hoshi, F., Higuchi, S., et al. Determination of canine β2-microgloblin in plasma and urine by enzyme-linked immunosorbent assay. J. Vet. Med. Sci. 63(3), 343-345 (2001).

    4. Tanaka, T., Shimada, T., Akiyoshi, H., et al. Germline polymorphism at the β2-microglobulin exon 1/intron 1 splice site in canine mammary gland simple and complex carcinomas. Vet. Rec. 172(20), 529 (2013).