A quantitative analytical standard guaranteed to meet MaxSpec® identity, purity, stability, and concentration specifications
Features
  • Purpose built for quantitative mass spectometry
  • Prepared gravimetrically as a solution with a defined concentration
  • Packaged in an ampule sealed under argon to maintain product integrity
  • Guaranteed to meet MaxSpec® identity, purity, stability, and concentration specifications
Related Products
Research Tool(s)
20849Cholic Acid-d4
Unlabeled Version(s)
31347Cholic Acid MaxSpec® Standard
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Cholic Acid-d4 MaxSpec® Standard

Item No. 31348

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3α,5β,7α,12α)-3,7,12-trihydroxy-cholan-24-oic-2,2,4,4-d4 acid
CAS Number
116380-66-6
Synonyms
  • CA-d4
  • Cholalic Acid-d4
  • Cholalin-d4
Molecular Formula
C24H36D4O5
Formula Weight
412.6
Purity
≥95%
Formulation (Request formulation change)
A solution in methanol at 100 µg/ml
SMILES
O[C@@H]1C([2H])([2H])C[C@@]2(C)[C@@](C[C@@H](O)[C@]3([H])[C@]2([H])C[C@H](O)[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(O)=O)([H])C1([2H])[2H]
InChi Code
InChI=1S/C24H40O5/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29)/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-/m1/s1/i8D2,10D2
InChi Key
BHQCQFFYRZLCQQ-YFEOEUIKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $143

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    Product Description

    Cholic acid-d4 is intended for use as an internal standard for the quantification of cholic acid (Item No. 20250) by GC- or LC-MS. Cholic acid is a primary bile acid.1 It is formed from cholesterol via a multistep process catalyzed by the cytochrome P450 (CYP) isoforms CYP7A1, CYP8B1, and CYP27A1. Cholic acid is conjugated to glycine or taurine by bile acid-CoA:amino acid N-acyltransferase (BAAT) to produce glycocholic acid (GCA; Item No. 20276) and taurocholic acid (TCA; Item No. 16215), respectively, in the liver, and is transformed into the secondary bile acid deoxycholic acid (DCA; Item No. 20756) by intestinal microbiota.1,2,3 It induces C. difficile colony formation in an agar dilution assay when used at a concentration of 0.1% w/v.4 Dietary administration of cholic acid (0.4% w/w) increases serum cholesterol levels, biliary phospholipid secretion, and fecal DCA levels in rats.5

    Cholic acid-d4 MaxSpec® standard is a quantitative grade standard of cholic acid-d4 (Item No. 20849) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This cholic acid4 MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Šarenac, T.M., and Mikov, M. Bile acid synthesis: From nature to the chemical modification and synthesis and their applications as drugs and nutrients. Front. Pharmacol. 9, 939 (2018).

    2. Hunt, M.C., Siponen, M.I., and Alexson, S.E.H. The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism. Biochim. Biophys. Acta 1822(9), 1397-1410 (2012).

    3. Staley, C., Weingarden, A.R., Khoruts, A., et al. Interaction of gut microbiota with bile acid metabolism and its influence on disease states. Appl. Microbiol. Biotechnol. 101(1), 47-64 (2017).

    4. Sorg, J.A., and Sonenshein, A.L. Bile salts and glycine as cogerminants for Clostridium difficile spores. J. Bacteriol. 190(7), 2505-2512 (2008).

    5. Uchida, K., Nomura, Y., and Takeuchi, N. Effects of cholic acid, chenodeoxycholic acid, and their related bile acids on cholesterol, phospholipid, and bile acid levels in serum, liver, bile, and feces of rats. J. Biochem. 87(1), 187-194 (1980).