For immunochemical detection of GAD65
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

GAD65 Monoclonal Antibody (Clone 9B10)

Item No. 31360

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • GAD2
  • GAD64
  • Glutamate Decarboxylase 2
  • Glutamate Decarboxylase 65 kDa Isoform
  • Glutamic Acid Decarboxylase 65
Immunogen
Full-length recombinant human GAD65
Clone Designation
9B10
MW
65.4 kDa
Formulation (Request formulation change)
100 µg of protein G affinity-purified antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, WB
Species Reactivity
(+) Human(+) Mouse
UniProt Accession №
Q05329
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $347

    Add
    Cayman Chemical
    Explore Obesity & Metabolic Disease Resources

    Discover high-quality research tools to investigate GLP-1 mechanisms and next-generation metabolic targets.

    OBESITY RESEARCH SOLUTIONS
    Product Description

    Glutamic acid decarboxylase 65 (GAD65), also known as GAD2, catalyzes the production of GABA from L-glutamic acid and is encoded by GAD2 in humans.1 It is primarily expressed in pancreatic islets but is also expressed in neurons of the central and peripheral nervous systems and is localized to vesicular membranes.1 Autoantibodies against GAD65 have been found in patients with type 1 diabetes mellitus or gestational diabetes mellitus and have been used as biomarkers of type 1 diabetes mellitus.1 GAD65 autoantibodies have also been found in patients with the rare neurological disorder stiff-man syndrome or autoimmune epilepsy.2,3 Cayman’s GAD65 Monoclonal Antibody (Clone 9B10) can be used for ELISA and Western blot (WB) applications. The antibody recognizes GAD65 at 65.4 kDa from human and mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Krueger, C., Stöcker, W., and Schlosser, M. Glutamic acid decarboxylase autoantibodies. Autoantibodies 369-378 (2007).

    2. McKeon, A., and Tracy, J.A. GAD65 neurological autoimmunity. Muscle Nerve 56(1), 15-27 (2017).

    3. Cabezudo-García, P., Mena-Vázquez, N., Villagrán-García, M., et al. Efficacy of antiepileptic drugs in autoimmune epilepsy: A systematic review. Seizure 59, 72-76 (2018).