A quantitative analytical standard guaranteed to meet MaxSpec® identity, purity, stability, and concentration specifications
Features
  • Purpose built for quantitative mass spectrometry
  • Prepared gravimetrically as a solution with a defined concentration
  • Packaged in an ampule sealed under argon to maintain product integrity
  • Guaranteed to meet MaxSpec® identity, purity, stability, and concentration specifications
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Glycochenodeoxycholic Acid MaxSpec® Standard

Item No. 31363

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(3α,5β,7α)-3,7-dihydroxy-24-oxocholan-24-yl]-glycine
CAS Number
640-79-9
Synonyms
  • GCDCA
Molecular Formula
C26H43NO5
Formula Weight
449.6
Purity
≥95%
Formulation (Request formulation change)
A solution in methanol at 100 µg/ml (nominal); see CofA for verified concentration
SMILES
O[C@@H]1CC[C@@]2(C)[C@@](C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(NCC(O)=O)=O)([H])C1
InChi Code
InChI=1S/C26H43NO5/c1-15(4-7-22(30)27-14-23(31)32)18-5-6-19-24-20(9-11-26(18,19)3)25(2)10-8-17(28)12-16(25)13-21(24)29/h15-21,24,28-29H,4-14H2,1-3H3,(H,27,30)(H,31,32)/t15-,16+,17-,18-,19+,20+,21-,24+,25+,26-/m1/s1
InChi Key
GHCZAUBVMUEKKP-GYPHWSFCSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    100 µg

    $75

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    Product Description

    Glycochenodeoxycholic acid (GCDCA) is a glycine-conjugated form of the primary bile acid chenodeoxycholic acid (Item No. 10011286).1 It reduces formation of cholic acid (Item No. 20250) in primary human hepatocytes when used at a concentration of 100 µM.2 GCDCA (50, 75, and 100 µM) reduces the number of LC3 puncta, a marker of autophagy, and is cytotoxic to L-02 hepatocytes.1 GCDCA (50 µM) induces apoptosis in isolated rat hepatocytes, an effect that can be blocked by the protein kinase C (PKC) inhibitor chelerythrine (Item No. 11314).3 Fecal levels of GCDCA are decreased in a rat model of high-fat diet-induced obesity compared with rats fed a normal diet.4

    Glycochenodeoxycholic acid MaxSpec® standard is a quantitative grade standard of glycochenodeoxycholic acid (Item No. 16942) that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. The verified concentration is provided on the certificate of analysis. This Glycochenodeoxycholic acid MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lan, W., Chen, Z., Chen, Y., et al. Glycochenodeoxycholic acid impairs transcription factor E3-dependent autophagy-lysosome machinery by disrupting reactive oxygen species homeostasis in L02 cells. Toxicol. Lett. 331, 11-21 (2020).

    2. Ellis, E., Axelson, M., Abrahamsson, A., et al. Feedback regulation of bile acid synthesis in primary human hepatocytes: Evidence that CDCA is the strongest inhibitor. Hepatology 38(4), 930-938 (2003).

    3. Gonzalez, B., Fisher, C., and Rosser, B.G. Glycochenodeoxycholic acid (GCDC) induced hepatocyte apoptosis is associated with early modulation of intracellular PKC activity. Mol. Cell. Biochem. 207(1-2), 19-27 (2000).

    4. Lin, H., An, Y., Tang, H., et al. Alterations of bile acids and gut microbiota in obesity induced by high fat diet in rat model. J. Agric. Food Chem. 67(13), 3624-3632 (2019).

    Product Citations

    Qu, P., Rom, O., Li, K., et al. DT-109 ameliorates nonalcoholic steatohepatitis in nonhuman primates. Cell Metab. 35(5), 742-757 (2023).