G6PDi-1

G6PDi-1 is a reversible and noncompetitive inhibitor of glucose-6-phosphate dehydrogenase (G6PDH; IC₅₀ = 0.07 µM), the enzyme that converts G6P to 6-phosphogluconolactone using NADP⁺ as a cofactor in the first step of the pentose phosphate pathway.¹ G6PDi-1 (10 and 50 µM) reduces NADPH levels in a variety of cultured cells, including red blood cells and T cells. It decreases colony formation of HCT116 cells when used at concentrations of 20 and 30 µM, an effect that can be rescued by the antioxidant N-acetyl cysteine. G6PDi-1 decreases the production of cytokines induced by phorbol 12-myristate 13-acetate (PMA; Item No. 10008014) and ionomycin (Item No. 10004974) in T cells and prevents PMA-induced oxidative burst in neutrophils.

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1. Ghergurovich, J.M., García-Cañaveras, J.C., Wang, J., et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat. Chem. Biol. 16(7), 731-739 (2020).