HVEM/CD270 Extracellular Domain (human, recombinant)

Herpes virus entry mediator (HVEM), also known as CD270, is a type I transmembrane glycoprotein encoded by TNFRSF14 in humans.^1,2 It is composed of an extracellular domain containing four cysteine-rich repeats (CRDs), a transmembrane domain, and a cytoplasmic domain, which facilitates intracellular signaling. HVEM is expressed on T and B cells, other hematopoietic cells, and mucosal epithelium.² It binds to B and T lymphocyte attenuator (BTLA), CD160, and LIGHT (Item No. 31841), also known as CD258, and induces co-activation or co-inhibition of T cells in a ligand-dependent manner via signaling through TNF receptor-associated factors (TRAFs) or inhibitory tyrosine-based motifs (ITIMs), respectively.^2,3 Tnfrsf14^-/- mice exhibit decreased susceptibility to bacterial infection, increased pro-inflammatory cytokine secretion and mortality in a model of concanavalin A-induced hepatitis, and increased susceptibility to the induction of experimental autoimmune encephalomyelitis (EAE), indicating roles for HVEM in both infection immunity and autoimmunity.⁴ Cayman’s HVEM/CD270 Extracellular Domain (human, recombinant) protein can be used for ELISA and enzyme activity assay applications. This protein consists of 177 amino acids and has a calculated molecular weight of 19 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 33 to 38 kDa due to glycosylation.

WARNING This product is not for human or veterinary use.

1. Connolly, S.A., Landsburg, D.J., Carfi, A., et al. Structure-based analysis of the herpes simplex virus glycoprotein D binding site present on herpesvirus entry mediator HveA (HVEM). J. Virol. 76(21), 10894-10904 (2002).

2. Ware, C.F. Targeting the LIGHT-HVEM pathway. Therapeutic Targets of the TNF Superfamily 146-155 (2009).

3. Shui, J.-W., and Kronenberg, M. HVEM: An unusual TNF receptor family member important for mucosal innate immune responses to microbes. Gut Microbes 4(2), 146-151 (2013).

4. Shui, J.-W., Steinberg, M.W., and Kronenberg, M. Regulation of inflammation, autoimmunity, and infection immunity by HVEM-BTLA signaling. J. Leukoc. Biol. 89(4), 517-523 (2011).