For immunochemical detection of IgG1
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IgG1 (mouse) Rabbit Monoclonal Antibody (Clone RM106)

Item No. 32003

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G1
Immunogen
Mouse IgG
Clone Designation
RM106
MW
~150 and 50 kDa for non-reduced and reduced mouse IgG1, respectively
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB
Cross Reactivity
(+) IgG1(-) Mouse IgG2a(-) Mouse IgG3(-) Mouse IgM(-) Mouse IgE(-) Human IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $411

    Add
    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 IgG1 production is driven by Th2-stimulated immune responses. IgG1 neutralizes toxins and activates the inhibitory FcγRIIB receptor but does not trigger FcγR-mediated effector functions. Formulations containing humanized, chimeric, or murine IgG1 monoclonal antibodies have been used in the treatment of inflammatory diseases, such as ulcerative colitis, multiple sclerosis, and asthma, as well as cancer.7 Cayman's IgG1 (mouse) Rabbit Monoclonal Antibody (Clone RM106) can be used for ELISA and Western blot (WB) applications. The antibody recognizes the Fc region of IgG1 and detects non-reduced and reduced IgG1 from mouse samples at approximately 150 and 50 kDa, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Collins, A.M. IgG subclass co-expression brings harmony to the quartet model of murine IgG function. Immunol. Cell Biol. 94(10), 949-954 (2016).

    6. Martin, R.M., Brady, J.L., and Lew, A.M. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J. Immunol. Methods 212(2), 187-192 (1998).

    7. Lu, R.-M., Hwang, Y.-C., Liu, I.-J., et al. Development of therapeutic antibodies for the treatment of diseases. J. Biomed. Sci. 27(1), 1 (2020).