For immunochemical detection of IgG1
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IgG1 (human) Rabbit Monoclonal Antibody - Biotinylated (Clone RM117)

Item No. 32373

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin IgG1
Immunogen
Peptide from the hinge region of human IgG1
Clone Designation
RM117
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, and IHC
Cross Reactivity
(+) IgG1(-) Human IgG2(-) Human IgG3(-) Human IgG4(-) Human IgM(-) Human IgA(-) Human IgD(-) Human IgE(-) Goat IgG(-) Mouse IgG(-) Rat IgG
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    50 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG1 binds to Fc receptors to induce Fc receptor-mediated effector functions, as well as Cq1 to induce complement activation in response to soluble and membrane protein antigens. Maternal IgG1s are shared with the fetus via placental transfer and children with group B streptococcal-induced sepsis are born to mothers with decreased serum levels of IgG1 compared with mothers of uninfected children.5 Cayman’s IgG1 (human) Rabbit Monoclonal Antibody - Biotinylated (Clone RM117) can be used for ELISA, immunocytochemistry (ICC), and immunohistochemistry (IHC) applications. The antibody recognizes the heavy chain of human IgG1.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Jefferis, R., and Kumararatne, D.S. Selective IgG subclass deficiency: Quantification and clinical relevance. Clin. Exp. Immunol. 81(3), 357-367 (1990).