Leukotriene B₄-d₄

Leukotriene B₄-d₄ (LTB₄-d₄) is intended for use as an internal standard for the quantification of LTB₄ (Item No. 20110) by GC- or LC-MS. LTB₄ is a dihydroxy fatty acid derived from arachidonic acid through the 5-lipoxygenase pathway.^1,2,3 It promotes a number of leukocyte functions including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis. In subnanomolar ranges (3.9 x 10⁻¹⁰ M), LTB₄ causes chemotaxis and chemokinesis in human PMNL.⁴ At higher concentrations, (1.0 x 10⁻⁷ M), LTB₄ leads to neutrophil aggregation and degranulation as well as superoxide anion production.^4,5

WARNING This product is not for human or veterinary use.

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2. Ford-Hutchinson, A.W., Bray, M.A., Doig, M.V., et al. Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature 286(5770), 264-265 (1980).

3. McGee, J., and Fitzpatrick, F. Enzymatic hydration of leukotriene A₄. Purification and characterization of a novel epoxide hydrolase from human erythrocytes. The Journal of Biological Chemisty 260(23), 12832-12837 (1985).

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