Soluble FLT3 Ligand (human, recombinant)

FMS-related tyrosine kinase 3 ligand (FLT3L) is a type I transmembrane protein that regulates hematopoietic stem and progenitor cell proliferation, differentiation, and survival.¹ It is composed of an N-terminal signaling peptide and extracellular cytokine domain, a transmembrane domain, and a cytosolic tail.² Soluble FLT3L is formed by proteolytic cleavage of membrane-bound FLT3L at the transmembrane domain or alternative splicing. Both membrane-bound and soluble FLT3L homodimerize and bind to FLT3 to induce ligand-dependent intracellular signaling and are expressed mainly by bone marrow stroma cells.^2,1 FLT3L synergizes with IL-7 to enhance B-lymphopoiesis and promotes IL-12-induced T cell production.³ It also acts on myeloid progenitor cells to induce proliferation of granulocytes and monocytes and increases the number of functional dendritic cells in a manner dependent on the presence of other growth factors such as GM-CSF (Item No. 32044), M-CSF, and IL-3. Flt3l knockdown induces hematopoietic developmental defects in mice. FLT3L induces proliferation and prevents apoptosis of acute myeloid leukemia cells in vitro. Cayman's Soluble FLT3 Ligand (human, recombinant) protein can be used for cell-based assay applications. This protein consists of 175 amino acids and has a calculated molecular weight of 20.2 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 27 kDa due to glycosylation.

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1. Naoe, T., and Kiyoi, H. Normal and oncogenic FLT3. Cell Mol. Life Sci. 61(23), 2932-2938 (2004).

2. Kazi, J., and Rönnstrand, L. FMS-like tyrosine kinase 3/FLT3: From basic science to clinical implications. Physiol. Rev. 99(3), 1433-1466 (2019).

3. Drexler, H.G., and Quentmeier, H. FLT3: Receptor and Ligand. Growth Factors 22(2), 71-73 (2004).