Nectin-2 Extracellular Domain (mouse, recombinant)

Nectin-2, also known as CD112, is a member of the nectin family of adhesion molecules that mediates the formation of adherens junctions and regulates immune cell activation.¹ It contains an N-terminal extracellular domain with three immunoglobulin-like (Ig-like) loops, C1-like and C2 domains that mediate dimerization, a transmembrane segment, and a C-terminal cytoplasmic tail that binds to the actin filament-binding protein afadin.^1,2 Nectin-2 is expressed by a variety of cell types, including epithelial cells, neurons, fibroblasts, Sertoli cells, and cancer cells, and is upregulated by TGF-β1 (Item No. 30606) or IFN-β stimulation.^2,3,1 It localizes to the cell surface where it forms cis-homodimers that associate by trans-interactions with cis-homodimers of other nectins and nectin-like molecules (Necls) expressed on adjacent cells, resulting in cell-cell adhesion.^4,1 Nectin-2 binds the co-stimulatory receptor CD226/DNAM-1, which is widely expressed by most immune cells, including T cells, B cells, natural killer (NK) cells, and monocytes, and the inhibitory receptors TIGIT and CD96, which are expressed by NK cells and T cells, thus regulating both the activation or inhibition of immune cells in a receptor-specific manner.¹ It is also the receptor for herpesvirus entry into cells. Sperm isolated from Nectin2^-/- mice have decreased motility and maturation and fail to fuse with oocytes, resulting in male-specific infertility.⁵ Neutralization of nectin-2 with a monoclonal antibody decreases tumor growth and reduces metastasis in OV-90 or MDA-MB-231 mouse xenograft models, respectively.² Cayman's Nectin-2 Extracellular Domain (mouse, recombinant) protein can be used for binding assay applications. This protein consists of 331 amino acids, has a calculated molecular weight of 36 kDa, and a predicted N-terminus of Gln32 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 40 to 45 kDa due to glycosylation.

WARNING This product is not for human or veterinary use.

1. Huang, K., and Lui, W.-Y. Nectins and nectin-like molecules (Necls): Recent findings and their role and regulation in spermatogenesis. Semin. Cell Dev. Biol. 59, 54-61 (2016).

2. Oshima, T., Sato, S., Kato, J., et al. Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers. Mol. Cancer 12, 60 (2013).

3. Takai, Y., Irie, K., Shimizu, K., et al. Nectins and nectin-like molecules: Roles in cell adhesion, migration, and polarization. Cancer Sci. 94(8), 655-667 (2003).

4. Brlić, P.K., Roviš, T.L., Cinamon, G., et al. Targeting PVR (CD155) and its receptors in anti-tumor therapy. Cell. Mol. Immunol. 16(1), 40-52 (2019).

5. Ogita, H., and Takai, Y. Cross-talk among integrin, cadherin, and growth factor receptor: Roles of nectin and nectin-like molecule. A Survey of Cell Biology 265, 1-54 (2008).