Neuregulin-1 Type I-β2b Extracellular Domain (human, recombinant; aa 20-238)

Neuregulin-1 type I-β2b is a trophic factor and member of the neuregulin-1 family of growth factors, which comprises greater than 30 isoforms grouped into types I through VI based on their N-terminal region, which is generated by alternative splicing of NRG1.¹ It is composed of a type I N-terminal domain, an immunoglobulin-like (Ig-like) domain, a glycosylated domain, an EGF-like domain, a linker region that contains a proteolytic cleavage site to release the mature protein, a transmembrane domain, and a cytoplasmic tail.^1,2 The type I-β2b splice variant of Nrg1 is mainly expressed in the brain and stomach but is also expressed at low levels in most tissues.² Neuregulin-1 signaling is mediated by homodimers of HER4 and the heterodimers HER2-HER3, HER2-HER4, HER3-HER4, and EGFR-HER4.¹ The cytoplasmic domain of neuregulin-1 type I-β2b induces apoptosis in breast cancer cells, and the mature protein induces apoptosis in fibroblasts in vitro.^3,2 This protein consists of 230 amino acids and has a calculated molecular weight of 25.3 kDa. By SDS-PAGE, the apparent molecular mass of the protein is approximately 45 kDa due to glycosylation.

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1. Mei, L., and Xiong, W.-C. Neuregulin 1 in neural development, synaptic plasticity and schizophrenia. Nat. Rev. Neurosci. 9(6), 437-452 (2008).

2. Grimm, S., and Leder, P. An apoptosis-inducing isoform of neu differentiation factor (NDF) identified using a novel screen for dominant, apoptosis-inducing genes. J. Exp. Med. 185(6), 1137-1142 (1997).

3. Weinstein, E.J., Grimm, S., and Leder, P. The oncogene heregulin induces apoptosis in breast epithelial cells and tumors. Oncogene 17(16), 2107-2113 (1998).