For immunochemical detection of histone H3K14Ac
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Histone H3K14Ac Monoclonal Antibody (RM130)

Item No. 32129

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H3 Lysine 14
Immunogen
An acetyl peptide corresponding to H3K14Ac
Clone Designation
RM130
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, Multiplex-based assays, ICC, ChIP, WB
Cross Reactivity
(+) H3K79Ac(-) Unmodified H3K79(-) H3K4Ac(-) H3K9Ac(-) H3K18Ac(-) H3K23Ac(-) H3K27Ac(-) H3K36Ac(-) H3K79Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail, that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Acetylation of histone H3 at lysine 14 (H3K14) is associated with transcriptional activation and is required for trimethylation of H3K4.3,4,5 Cayman's Histone H3K14Ac Monoclonal Antibody (RM130) can be used for ELISA, multiplex-based assay, immunocytochemistry (ICC), chromatin immunoprecipitation (ChIP), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Gatta, R., and Mantovani, R. Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters. Cell Cycle 9(11), 2149-2159 (2010).

    4. Keating, S.T., van Diepen, J.A., Risken, N.P., et al. Epigenetics in diabetic nephropathy, immunity and metabolism. Diabetologia 61(1), 6-20 (2018).

    5. Nakanishi, S., Sanderson, B.W., Delventhal, K.M., et al. A comprehensive library of histone mutants identifies nucleosomal residues required for H3K4 methylation. Nat. Struct. Mol. Biol. 15(8), 881-888 (2008).