For immunochemical detection of H3K79Ac
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Histone H3K79Ac Monoclonal Antibody (RM156)

Item No. 32136

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H3 Lysine 79
Immunogen
Peptide corresponding to H3K79Ac
Clone Designation
RM156
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H3K79Ac(-) H3K4Ac(-) H3K9Ac(-) H3K14Ac(-) H3K18Ac(-) H3K23Ac(-) H3K27Ac(-) H3K36Ac(-) H3K56Ac(-) H3K122Ac(-) Unmodified H3K79
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Acetylation of histone H3 at lysine 79 (H3K79Ac) has been detected in humans and yeast and is associated with inactive chromatin.3,4,5 Cayman’s Histone H3K79Ac Monoclonal Antibody (RM156) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Garcia, B.A., Hake, S.B., Diaz, R.L., et al. Organismal differences in post-translational modifications in histones H3 and H4. The Journal of Biological Chemisty 282(10), 7641-7655 (2007).

    4. Bheda, P., Swatkoski, S., Fiedler, K.L., et al. Biotinylation of lysine method identifies acetylated histone H3 lysine 79 in Saccharomyces cerevisiae as a substrate for Sir2. Proc. Natl. Acad. Sci. USA 109(16), E916-E925 (2012).

    5. Gatta, R., and Mantovani, R. Single nucleosome ChIPs identify an extensive switch of acetyl marks on cell cycle promoters. Cell Cycle 9(11), 2149-2159 (2010).