For immunochemical detection of H3K9Me3
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Histone H3K9Me3 Monoclonal Antibody (RM389)

Item No. 32138

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Trimethylated Histone H3 Lysine 9
Immunogen
Peptide corresponding to H3K9Me3
Clone Designation
RM389
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H3K9Me3(-) Unmodified H3K9(-) H3K9Me1(-) H3K9Me2(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H4K20Me1(-) H4K20Me2(-) H4K20Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of histone H3 at lysine 9 (H3K9Me3) occludes DNA binding by transcription factors and is associated with gene repression.3 H3K9Me3 is also a binding target of the heterochromatin protein 1 (HP1) family proteins CBX1, CBX3, and CBX5.4 It is recognized by the plant homeodomain (PHD) fingers of chromodomain helicase DNA binding protein 4 (CHD4), a member of the NuRD deacetylase complex that is upregulated in a variety of cancers and implicated in the DNA damage response.5 Cayman's Histone H3K9Me3 Monoclonal Antibody (RM389) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Becker, J.S., Nicetto, D., and Zaret, K.S. H3K9me3-dependent heterochromatin: Barrier to cell fate changes. Trends Genet. 32(1), 29-41 (2016).

    4. Kaustov, L., Ouyang, H., Amaya, M., et al. Recognition and specificity determinants of the human Cbx chromodomains. The Journal of Biological Chemisty 286(1), 521-529 (2011).

    5. Mansfield, R.E., Musselman, C.A., Kwan, A.H., et al. Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9. The Journal of Biological Chemisty 286(13), 11779-11791 (2011).