For immunochemical detection of H3K9Ac
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Histone H3K9Ac Monoclonal Antibody (RM161)

Item No. 32139

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H3 Lysine 9
Immunogen
Peptide corresponding to H3K9Ac
Clone Designation
RM161
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, IHC, multiplex-based assays, WB
Cross Reactivity
(+) H3K9Ac(-) H3K4Ac(-) H3K14Ac(-) H3K18Ac(-) H3K23Ac(-) H3K27Ac(-) H3K36Ac(-) H3K56Ac(-) H3K79Ac(-) H3K122Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Acetylation of histone H3 at lysine 9 (H3K9Ac) is associated with active gene transcription and can recruit the super elongation complex to chromatin through direct binding with the AF9 and ENL subunits.3,4 It increases at the IFN-β promoter in HeLa cells upon infection with Sendai virus.5 Acetylation of histone H3 at lysine 9 decreases following induction of DNA damage in HeLa and U2OS cells.6 Cayman's Histone H3K9Ac Monoclonal Antibody (RM161) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Jenuwein, T., and Allis, C.D. Translating the histone code. Science 293(5532), 1074-1080 (2001).

    4. Gates, L.A., Shi, J., Rohira, A.D., et al. Acetylation on histone H3 lysine 9 mediates a switch from transcription initiation to elongation. The Journal of Biological Chemisty 292(35), 14456-14472 (2017).

    5. Agalioti, T., Chen, G., and Thanos, D. Deciphering the transcriptional histone acetylation code for a human gene. Cell 111(3), 381-392 (2002).

    6. Tjeertes, J.V., Miller, K.M., and Jackson, S.P. Screen for DNA-damage-responsive histone modifications identifies H3K9Ac and H3K56Ac in human cells. EMBO J. 28(13), 1878-1889 (2009).