For immunochemical detection of H3K27Ac
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Histone H3K27Ac Monoclonal Antibody (RM172)

Item No. 32141

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H3 Lysine 27
Immunogen
Peptide corresponding to H3K27Ac
Clone Designation
RM172
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H3K27Ac(-) Unmodified H3K27(-) H3K4Ac(-) H3K9Ac(-) H3K14Ac(-) H3K18Ac(-) H3K23Ac(-) H3K36Ac(-) H3K56Ac(-) H3K79Ac(-) H3K122Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Acetylation of histone H3 at lysine 27 (H3K27Ac) is associated with active gene transcription.3 H3K27 acetylation and H3K4 monomethylation are enriched near active enhancers, and H3K27 acetylation and H3K4 trimethylation together mark transcription start sites of actively transcribed genes. Increased tumor H3K27Ac levels have been found in patients with breast cancer.4 Cayman's Histone H3K27Ac Monoclonal Antibody (RM172) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Kimura, H. Histone modifications for human epigenome analysis. J. Hum. Genet. 58(7), 439-445 (2013).

    4. Li, Q.-L., Wang, D.-Y., Ju, L.-G., et al. The hyper-activation of transcriptional enhancers in breast cancer. Clin. Epigenetics 11(1), 48 (2019).