For immunochemical detection of H3K14Me2
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Histone H3K14Me2 Monoclonal Antibody (RM165)

Item No. 32143

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dimethylated Histone H3 Lysine 14
Immunogen
Peptide corresponding to H3K14Me2
Clone Designation
RM165
Formulation (Request formulation change)
100 µg of protein A affinity-purified monoclonal antibody
Storage Buffer
PBS with 50% Glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, and WB
Cross Reactivity
(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me1(+) H3K14Me2(-) H3K14Me3(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1(-) H3K79Me1(-) H3K79Me2(-) H3K79Me3(-) Unmodified H3K14
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone 3 lysine 14 (H3K14Me2) is enriched in the promoter region of tumor suppressor candidate 3 (TUSC3), but not that of GAPDH, in DLD-1 and HCT116 cells.3 Ubiquitin-like PHD and RING finger domain-containing protein 1 (UHRF1) can bind to dimethylated H3K14 and suppress acetylation of H3K14. H3K14Me2 levels increase during transdifferentiation of rat Thy-1(+) Lin(-) bone marrow cells into hepatocytes in vitro.4 Cayman’s Histone H3K14Me2 Monoclonal Antibody (RM165) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Taniue, K., Hayashi, T., Kamoshida, Y., et al. UHRF1-KAT7-mediated regulation of TUSC3 expression via histone methylation/acetylation is critical for the proliferation of colon cancer cells. Oncogene 39(5), 1018-1030 (2019).

    4. Liao, X., Liao, Y., Zou, Y., et al. Epigenetic modifications of histone H3 during the transdifferentiation of Thy-1(+) Lin(‑) bone marrow cells into hepatocytes. Mol. Med. Rep. 15(5), 7561-7567 (2015).