For immunochemical detection of histone H3K4Ac
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Histone H3K4Ac Monoclonal Antibody (RM149)

Item No. 32144

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H3 Lysine 4
Immunogen
Peptide corresponding to H3K4Ac
Clone Designation
RM149
Formulation (Request formulation change)
100 µg of protein A-purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H3K4Ac(-) Unmodified histone H3 (1-19)(-) H3K9Ac(-) H3K14Ac(-) H3K18Ac(-) H3K23Ac(-) H3K27Ac(-) H3K36Ac(-) H3K56Ac(-) H3K79Ac(-) H3K122Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Acetylation of histone H3 at lysine 4 (H3K4Ac) occurs primarily at the promoter and transcribed regions of active genes.3 H3K4Ac is enriched at the promoters of genes involved in the estrogen response and epithelial-to-mesenchymal transition (EMT) in MCF-7 and MDA-MB-231 breast cancer cells.4 Cayman's Histone H3K4Ac Monoclonal Antibody (RM149) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Wang, Z., Zang, C., Rosenfeld, J.A., et al. Combinatorial patterns of histone acetylations and methylations in the human genome. Nat. Genet. 40(7), 897-903 (2008).

    4. Messier, T.L., Gordon, J.A.R., Boyd, J.R., et al. Histone H3 lysine 4 acetylation and methylation dynamics define breast cancer subtypes. Oncotarget 7(5), 5094-5109 (2016).