For immunochemical detection of H3K18Me2
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Histone H3K18Me2 Monoclonal Antibody (RM168)

Item No. 32148

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dimethylated Histone H3 Lysine 18
Immunogen
Peptide corresponding to H3K18Me2
Clone Designation
RM168
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H3K18Me2(-) Unmodified H3K18(-) H3K4Me1(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me3(-) H3K56Me1(-) H3K79Me1(-) H3K79Me2(-) H3K79Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
    Explore Obesity & Metabolic Disease Resources

    Discover high-quality research tools to investigate GLP-1 mechanisms and next-generation metabolic targets.

    OBESITY RESEARCH SOLUTIONS
    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Histone H3 lysine 18 (H3K18) is subject to both acetylation and methylation.3 An H3K18Me2 (1-21) peptide reduces the association of lamin A/C with chromatin in a chromatin immunoprecipitation (ChIP) assay.4 Dimethylation of histone H3 at lysine 18 is increased in patients with diabetes.5 Cayman’s Histone H3K18Me2 Monoclonal Antibody (RM168) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Garcia, B.A., Busby, S.A., Shabanowitz, J., et al. Resetting the epigenetic histone code in the MRL-lpr/lpr mouse model of lupus by histone deacetylase inhibition. J. Proteome Res. 4(6), 2032-2042 (2005).

    4. Vazquez, B.N., Thackray, J.K., Simonet, N.G., et al. SIRT7 mediates L1 elements transcriptional repression and their association with the nuclear lamina. Nucleic Acids Res. 47(15), 7870-7885 (2019).

    5. Wang, W., Sidoli, S., Zhang, W., et al. Abnormal levels of histone methylation in the retinas of diabetic rats are reversed by minocycline treatment. Sci. Rep. 7, 45103 (2017).