For immunochemical detection of H3K9Me2/K14Ac
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Histone H3K9Me2/K14Ac Monoclonal Antibody (RM322)

Item No. 32152

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Peptide corresponding to H3K9Me2/K14Ac
Clone Designation
RM322
Formulation (Request formulation change)
100 µg of Protein A affinity-purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
Multiplex-based assays and WB
Cross Reactivity
(+) H3K9Me2/K14Ac(-) Unmodified histone H3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K9Ac(-) H3K14Ac(-) H3K14Me2
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression, whereas acetylation of histone H3 at lysine 14 (H3K14Ac) is associated with transcriptional activation.3,4 H3K9Me2/K14Ac levels are increased in mouse differentiated embryonic stem cells (ESCs) compared with undifferentiated ESCs.5 Altered H3K9Me2/K14Ac levels have been found in tumor biopsies from patients with HER2+, triple-negative, luminal A-like, or luminal B-like breast cancer.6 Cayman's Histone H3K9Me2/K14Ac Monoclonal Antibody (RM322) recognizes histone H3 only when modified by both dimethylation at K9 and acetylation at K14. This antibody can be used for multiplex-based assay and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Lienert, F., Mohn, F., Tiwari, V.K., et al. Genomic prevalence of heterochromatic H3K9me2 and transcription do not discriminate pluripotent from terminally differentiated cells. PLoS Genet. 7(6), e1002090 (2011).

    4. Keating, S.T., van Diepen, J.A., Risken, N.P., et al. Epigenetics in diabetic nephropathy, immunity and metabolism. Diabetologia 61(1), 6-20 (2018).

    5. Gonzales-Cope, M., Sidoli, S., Bhanu, N.V., et al. Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells. BMC Genomics. 17, 95 (2016).

    6. Noberini, R., Uggetti, A., Pruneri, G., et al. Pathology tissue-quantitative mass spectrometry analysis to profile histone post-translational modification patterns in patient samples. Mol. Cell Proteomics 15(3), 866-877 (2016).