For immunochemical detection of H4K5Ac
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Histone H4K5Ac Monoclonal Antibody (RM199)

Item No. 32156

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H4 Lysine 5
Immunogen
Peptide corresponding to H4K5Ac
Clone Designation
RM199
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H4K5Ac(-) Unmodified H4K5(-) H4K8Ac(-) H4K12Ac(-) H4K16Ac(-) H4K20Ac(-) H4K31Ac(-) H4K91Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of posttranslational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Acetylation of histone H4 at lysine 5 (H4K5Ac) is enriched in promoter regions and coding sequences in mouse hippocampus and is associated with active chromatin.3,4 Hippocampal levels of H4K5Ac increase following contextual fear conditioning in mice.3 Levels of H4K5Ac are elevated in postmortem brain tissue isolated from fetuses with spina bifida compared with healthy controls.5 Global levels of H4K5Ac are decreased in blast cells from patients with acute myeloid leukemia compared with healthy individuals, and low levels of H4K5Ac are associated with poor prognosis in these patients.6 Cayman's Histone H4K5Ac Monoclonal Antibody (RM199) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Park, C.S., Rehrauer, H., and Mansuy, I.M. Genome-wide analysis of H4K5 acetylation associated with fear memory in mice. BMC Genomics 14, 539 (2013).

    4. Zhao, R., Nakamura, T., Fu, Y., et al. Gene bookmarking accelerates the kinetics of post-mitotic transcriptional re-activation. Nat. Cell Biol. 13(11), 1295-1304 (2011).

    5. Li, D., Wan, C., Bai, B., et al. Identification of histone acetylation markers in human fetal brains and increased H4K5ac expression in neural tube defects. Mol. Genet. Genomic Med. 7(12), e1002 (2019).

    6. Sauer, T., Arteaga, M.F., Isken, F., et al. MYST2 acetyltransferase expression and Histone H4 Lysine acetylation are suppressed in AML. Exp. Hematol. 43(9), 794-802 (2015).