For immunochemical detection of H4K8Ac
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Histone H4K8Ac Monoclonal Antibody (RM201)

Item No. 32157

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H4 Lysine 8
Immunogen
Peptide corresponding to H4K8Ac
Clone Designation
RM201
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% Glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, and WB
Cross Reactivity
(+) H4K8Ac(-) Unmodified H4K8(-) H4K5Ac(-) H4K12Ac(-) H4K16Ac(-) H4K20Ac(-) H4K31Ac(-) H4K91Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Acetylation of histone H4 at lysine 8 (H4K8Ac) is associated with transcriptional activation and mediates recruitment of SWI/SNF chromatin remodeling complexes.3,4 P. falciparum schizonts expressing a lysine-to-arginine substitution at Lys8 in histone 4, which prevents its acetylation, have increased multiplication rates.5 Tumor H4K8Ac levels are increased in patients with breast cancer.6 Cayman's Histone H4K8Ac Monoclonal Antibody (RM201) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Keating, S.T., van Diepen, J.A., Risken, N.P., et al. Epigenetics in diabetic nephropathy, immunity and metabolism. Diabetologia 61(1), 6-20 (2018).

    4. Agalioti, T., Chen, G., and Thanos, D. Deciphering the transcriptional histone acetylation code for a human gene. Cell 111(3), 381-392 (2002).

    5. Gupta, A.P., Zhu, L., Tripathi, J., et al. Histone 4 lysine 8 acetylation regulates proliferation and host-pathogen interaction in Plasmodium falciparum. Epigenetics Chromatin 10(1), 40 (2017).

    6. Li, Q.-L., Wang, D.-Y., Ju, L.-G., et al. The hyper-activation of transcriptional enhancers in breast cancer. Clin. Epigenetics 11(1), 48 (2019).