Histone H4K16Ac Monoclonal Antibody (RM204)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.¹ Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.^1,2 Acetylation of histone H4 at lysine 16 (H4K16Ac) is associated with transcriptional activation.³ Cellular H4K16Ac levels are decreased in Jurkat and HL-60 leukemia cells compared with normal human lymphocytes, and tumor H4K16Ac levels are reduced in patients with lymphoma or colorectal cancer.⁴ Cayman's Histone H4K16Ac Monoclonal Antibody (RM204) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

3. Keating, S.T., van Diepen, J.A., Risken, N.P., et al. Epigenetics in diabetic nephropathy, immunity and metabolism. Diabetologia 61(1), 6-20 (2018).

4. Fraga, M.F., Ballestar, E., Villar-Garea, A., et al. Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer. Nat. Genet. 37(4), 391-400 (2005).