For immunochemical detection of H4K12Ac
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Histone H4K12Ac Monoclonal Antibody (RM202)

Item No. 32162

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H4 Lysine 12
Immunogen
Peptide corresponding to H4K12Ac
Clone Designation
RM202
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H4K12Ac(-) Unmodified H4K12(-) H4K5Ac(-) H4K8Ac(-) H4K16Ac(-) H4K20Ac(-) H4K31Ac(-) H4K91Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Acetylation of histone H4 at lysine 12 (H4K12Ac) is associated with chromatin relaxation and gene transcription.3 Levels of H4K12Ac are elevated in two transgenic mouse models of Alzheimer's disease, as well as in monocytes isolated from patients with mild cognitive impairment and Alzheimer's disease. H4K12Ac levels are also increased in response to chronic alcohol exposure and positively correlate with the release of the pro-inflammatory cytokine chemokine (C-C motif) ligand 8 (CCL8) in monocyte-derived dendritic cells.4 Cayman's H4K12Ac Monoclonal Antibody (RM202) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Plagg, B., Ehrlich, D., Kniewallner, K.M., et al. Increased acetylation of histone H4 at lysine 12 (H4K12) in monocytes of transgenic Alzheimer’s mice and in human patients. Curr. Alzheimer Res. 12(8), 752-760 (2015).

    4. Parira, T., Figueroa, F., Laverde, A., et al. Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci. Rep. 7(1), 11236 (2017).