For immunochemical detection of H2BK20Ac
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Histone H2BK20Ac Monoclonal Antibody (Clone RM235)

Item No. 32173

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Acetylated Histone H2B Lysine 20
Immunogen
Peptide corresponding to H2BK20Ac
Clone Designation
RM235
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H2BK20Ac(-) Unmodified H2BK20(-) H2BK5Ac(-) H2BK11Ac(-) H2BK12Ac(-) H2BK15Ac(-) H2BK16Ac(-) H2BK23Ac
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H2B is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.1,2,3 Acetylation of histone H2B at lysine 20 (H2BK20Ac) at enhancer regions serves as a hallmark of active enhancers.4 H2BK20Ac is enriched at cell type-specific promoter regions and is required for targeting of the histone variant macroH2A1 to chromatin containing acetylated histone H2B.4,5 Cayman's Histone H2BK20Ac Monoclonal Antibody (Clone RM235) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

    3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

    4. Kumar, V., Rayan, N.A., Muratani, M., et al. Comprehensive benchmarking reveals H2BK20 acetylation as a distinctive signature of cell-state-specific enhancers and promoters. Genome Res. 26(5), 612-623 (2016).

    5. Ruiz, P.D., and Gamble, M.J. MacroH2A1 chromatin specification requires its docking domain and acetylation of H2B lysine 20. Nat. Commun. 9(1), 5143 (2018).