For immunochemical detection of H3K9Cr
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Histone H3K9Cr Monoclonal Antibody (Clone RM339)

Item No. 32177

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Crotonylated Histone H3 Lysine 9
Immunogen
Peptide corresponding to H3K9Cr
Clone Designation
RM339
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
Dot blot, multiplex-based assay, WB
Cross Reactivity
(+) H3K9Cr(+) H3K9Bu(+) H3K9Pr (weakly)(-) Unmodified histone H3(-) H3K9Ac(-) H3K9βhb
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, citrullination, and crotonylation.1,2,3 Crotonylation of histone H3 at lysine 9 (H3K9Cr) is regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs).4 H3K9Cr binds the YEATS domain of human AF9 and yeast Taf14 and is associated with transcriptional activation.4,5 H3K9Cr levels are reduced by DNA damage in U2OS cancer cells.6 Cayman's H3K9Cr Monoclonal Antibody (Clone RM339) can be used for dot blot, multiplex-based assay, and Western blot (WB) applications. This antibody is cross-reactive with histone H3 butyrylated at lysine 9 (H3K9Bu).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Wan, J., Liu, H., Chu, J., et al. Functions and mechanisms of lysine crotonylation. J. Cell. Mol. Med. 23(11), 7163-7169 (2019).

    4. Andrews, F.H., Shinsky, S.A., Shanle, E.K., et al. The Taf14 YEATS domain is a reader of histone crotonylation. Nat. Chem. Biol. 12(6), 396-398 (2016).

    5. Li, Y., Sabari, B.R., Panchenko, T., et al. Molecular coupling of histone crotonylation and active transcription by AF9 YEATS domain. Mol. Cell 62(2), 181-193 (2016).

    6. Abu-Zhayia, E.R., Machour, F.E., and Ayoub, N. HDAC-dependent decrease in histone crotonylation during DNA damage. J. Mol. Cell Biol. 11(9), 804-806 (2019).