For immunochemical detection of mTOR
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mTOR Rabbit Monoclonal Antibody (Clone RM274)

Item No. 32224

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Mammalian Target of Rapamycin
  • Mechanistic Target of Rapamycin Kinase
  • RAFT1
  • RAPT1
Immunogen
Peptide corresponding to human mTOR
Clone Designation
RM274
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) mTOR
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Mammalian target of rapamycin (mTOR) is a serine/threonine kinase and member of the PI3K-related kinase family with roles in the regulation of cell growth and metabolism.1,2 It is comprised of N-terminal tandemly repeated HEAT motifs that facilitate protein-protein interactions, a FRAP, ATM, and TRRAP (FAT) domain, an FKBP12-rapamycin binding (FRB) domain, a catalytic kinase domain, an autoinhibitory/repressor domain, and a FAT carboxy-terminal (FATC) domain that is essential to kinase activity.2 mTOR is ubiquitously expressed in the cytosol and is the catalytic subunit of mTOR complex 1 (mTORC1) and mTORC2, which have roles in aging, autophagy, stem cell and immune function, cellular senescence, and macromolecule biogenesis, and cell survival, cytoskeletal organization, and metabolism, respectively.2,3 It is a component of the PI3K/AKT/mTOR signaling pathway that acts as a junction point both upstream and downstream of AKT.4 Dysregulation of the mTOR signaling pathway is associated with various pathologies, including cancer, rheumatoid arthritis, epilepsy, neurodegenerative diseases, and diabetes.4,5,2 Cayman’s mTOR Rabbit Monoclonal Antibody (Clone RM274) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weichhart, T. mTOR as regulator of lifespan, aging, and cellular senescence: A mini-review. Gerontology 64(2), 127-134 (2018).

    2. Showkat, M., Beigh, M.A., and Andrabi, K.I. mTOR signaling in protein translation regulation: Implications in cancer genesis and therapeutic interventions. Mol. Biol. Int. 686984 (2014).

    3. Zarogoulidis, P., Lampaki, S., Turner, J.F., et al. mTOR pathway: A current, up-to-date mini-review (review). Oncol. Lett. 8(6), 2367-2370 (2014).

    4. Laplante, M., and Sabatini, D.M. mTOR signaling at a glance. J. Cell Sci. 122(pt 20), 3589-3594 (2009).

    5. Wong, M. A critical review of mTOR inhibitors and epilepsy: From basic science to clinical trials. Expert Rev. Neurother. 13(6), 657-669 (2013).