For immunochemical detection of β-catenin
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Alternative(s)
100029β-Catenin Polyclonal Antibody
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β-Catenin Rabbit Monoclonal Antibody (Clone RM276)

Item No. 32226

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Catenin β-1
  • Catenin (Cadherin-associated Protein), β1
  • CTNNB1
  • EVR7
  • MRD19
  • NEDSDV
Immunogen
Peptide corresponding to human β-Catenin
Clone Designation
RM276
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) β-Catenin
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    β-Catenin is a transcriptional coactivator that is encoded by the CTNNB1 gene in humans.1,2 It is a 781 amino acid protein comprised of an N-terminal domain containing glycogen synthase kinase 3β (GSK3β) phosphorylation sites, a C-terminal transactivation domain, and a central domain spanning amino acid residues 138-664.3,4 The central domain consists of 12 armadillo repeats and is required for binding to cadherins, TCF/LEF transcription factors, and adenomatous polyposis coli (APC). β-Catenin has roles in cell adhesion, canonical Wnt signaling, regulation of stem cells, embryonic development, and adult tissue homeostasis, among others.1,3 In the absence of Wnt, a complex consisting of axin, APC, GSK3β, and casein kinase 1 (CK1), binds to and phosphorylates β-catenin, targeting it for ubiquitination and proteosomal degradation.1 In the presence of Wnt, phosphorylation of β-catenin is inhibited, allowing β-catenin to translocate into the nucleus, where it interacts with TCF/LEF to activate expression of Wnt target genes. Activating mutations in CTNNB1 that stabilize β-catenin have been associated with a variety of cancers, including hepatocellular and adrenocortical carcinomas, colorectal cancer, and pilomatricomas.4,5,6,7 Cayman's β-Catenin Rabbit Monoclonal Antibody (Clone RM276) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. MacDonald, B.T., Tamai, K., and He, X. Wnt/β-catenin Signaling: Components, mechanisms, and diseases. Dev. Cell. 17(1), 9-26 (2009).

    2. Nollet, F., Berx, G., Molemans, F., et al. Genomic organization of the human β-catenin gene (CTNNB1). Genomics 32(3), 413-424 (1996).

    3. Xing, Y., Takemaru, K.-I., Liu, J., et al. Crystal structure of a full-length β-catenin. Structure 16(3), 478-487 (2008).

    4. Akiyama, T. Wnt/β-catenin signaling. Cytokine Growth Factor Rev. 11(4), 273-282 (2000).

    5. de La Coste, A., Romagnolo, B., Billuart, P., et al. Somatic mutations of the β-catenin gene are frequent in mouse and human hepatocellular carcinomas. Proc. Natl. Acad. Sci. USA 95(15), 8847-8851 (1998).

    6. Clevers, H. Wnt/β-catenin signaling in development and disease. Cell 127(3), 469-480 (2006).

    7. Durand, J., Lampron, A., Mazzuco, T.L., et al. Characterization of differential gene expression in adrenocortical tumors harboring β-catenin (CTNNB1) mutations. J. Clin. Endocrinol. Metab. 96(7), E1206-E1211 (2011).