For immunochemical detection of cytokeratin 18
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Cytokeratin 18 Rabbit Monoclonal Antibody (Clone RM279)

Item No. 32229

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CK-18
  • Keratin-18
  • Keratin, Type II Cytoskeletal 18
  • KRT18
Immunogen
Peptide from the N-terminal region of human cytokeratin 18
Clone Designation
RM279
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Cytokeratin 18
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    Cytokeratin 18 (CK18) is an intermediate filament protein and a member of the cytokeratin protein family.1 It is a type I acidic cytokeratin expressed in epithelial tissues, including those found in liver, lung, kidney, pancreas, and the gastrointestinal tract, and is localized in the cytoplasm and perinuclear region. CK18 is co-expressed with the type II neutral-basic cytokeratin CK8, with which it forms intermediate filaments essential to cytoplasmic structure. During apoptosis, CK18 is cleaved by caspases and released from the cell, and is commonly used as a marker of epithelial cell death.2 Plasma levels of CK18 positively correlate with the magnitude of hepatocyte apoptosis in patients with non-alcoholic fatty liver disease (NAFLD) and are independently predictive of non-alcoholic steatohepatitis (NASH).3 Serum levels of CK18 are elevated in patients with cardiometabolic disorders, including diabetes and hypertension, in an NAFLD-independent manner.2 CK18 tumor expression positively correlates with poorly differentiated oral squamous cell carcinoma (OSCC) tumors and poor prognosis.4 Cayman’s Cytokeratin 18 Rabbit Monoclonal Antibody (Clone RM279) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weng, Y.-R., Cui, Y., and Fang, J.-Y. Biological functions of cytokeratin 18 in cancer. Mol. Cancer Res. 10(4), 485-493 (2012).

    2. Qian, L., Zhang, L., Wu, L., et al. Elevated serum level of cytokeratin 18 M65ED is an independent indicator of cardiometabolic disorders. J. Diabetes Res. 5198359 (2020).

    3. Feldstein, A.E., Wieckowska, A., Lopez, A.R., et al. Cytokeratin-18 fragment levels as noninvasive biomarkers for nonalcoholic steatohepatitis: A multicenter validation study. Hepatology 50(4), 1072-1078 (2009).

    4. Makino, T., Yamasaki, M., Takeno, A., et al. Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus. Br. J. Cancer 101(8), 1298-1306 (2009).