For immunochemical detection of GluR1 (phospho-Ser845)
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

GluR1 (Phospho-Ser845) Rabbit Monoclonal Antibody (Clone RM296)

Item No. 32242

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • AMPA-selective Glutamate Receptor 1
  • GluA1
  • GluR-1
  • GluR-A
Immunogen
Peptide corresponding to human GluR1 (phospho-Ser845)
Clone Designation
RM296
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) GluR1 (phospho-Ser845)(-) GluR1 without phosphorylation at Ser845
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Product Description

    GluR1 is a subunit of the AMPA ionotropic glutamate receptor, which is responsible for fast excitatory synaptic transmission in the CNS.1 AMPA receptors are composed of four subunits, GluR1, GluR2, GluR3, and GluR4, which combine into heterotetramers to form a cation-permeable pore in the plasma membrane. Each subunit has two isoforms with the primary isoform designated as flip and a second isoform generated through alternative splicing designated as flop.2,3 The GluR1 flip and flop isoforms do not affect desensitization or channel opening and closing kinetics.3 GluR1 can be phosphorylated by PKA at serine 845 (Ser845), which increases the peak open probability of the ion channel, and dephosphorylation is required for AMPA receptor endocytosis.1 GluR1 (phospho-Ser845) levels increase in the ventromedial prefrontal cortex (vmPFC) and nucleus accumbens (NAc) following cocaine-cue extinction.4 Levels of GluR1 (phospho-Ser845) are reduced following NMDA receptor activation in rat hippocampal slices and by amyloid-β (Aβ) oligomers in primary mouse neurons.5,6 Cayman’s GluR1 (Phospho-Ser845) Rabbit Monoclonal Antibody (Clone RM296) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Banke, T.G., Bowie, D., Lee, H.-K., et al. Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase. J. Neurosci. 20(1), 89-102 (2000).

    2. Sommer, B., Keinänen, K., Verdoorn, T.A., et al. Flip and flop: A cell-specific functional switch in glutamate-operated channels of the CNS. Science 249(4976), 1580-1585 (1990).

    3. Pei, W., Huang, Z., Wang, C., et al. Flip and flop: A molecular determinant for AMPA receptor channel opening. Biochemistry 48(17), 3767-3777 (2009).

    4. Dhonnchadha, B.Á.N., Lin, A., Leite-Morris, K.A., et al. Alterations in expression and phosphorylation of GluA1 receptors following cocaine-cue extinction learning. Behav. Brain Res. 238, 119-123 (2013).

    5. Ai, H., Yang, W., Ye, M., et al. Differential regulation of AMPA receptor GluA1 phosphorylation at serine 831 and 845 associated with activation of NMDA receptor subpopulations. Neurosci. Lett. 497(2), 94-98 (2011).

    6. Miñano-Molina, A.J., España, J., Martín, E., et al. Soluble oligomers of amyloid-β peptide disrupt membrane trafficking of α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor contributing to early synapse dysfunction. The Journal of Biological Chemisty 286(31), 27311-27321 (2011).