For immunochemical detection of AMACR
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AMACR (N-Term) Rabbit Monoclonal Antibody (Clone RM349)

Item No. 32274

Product Insert (PDF)
Technical Information
Synonyms
  • 2-arylpropionyl-CoA Epimerase
  • 2-R-Ibuprofenyl racemase
  • α-methylacyl-CoA
  • P504S
Immunogen
Peptide from the N-terminal region of human AMACR
Clone Designation
RM349
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) AMACR
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Product Description

    α-methylacyl-Coenzyme A (α-methylacyl-CoA) racemase (AMACR) is an enzyme involved in the metabolism of branched chain fatty acids and 2-arylpropanoic acids (2-APAs), such as ibuprofen (Item No. 70280).1 It is expressed in most human tissues and is localized to peroxisomes and mitochondria.1,2 AMACR converts R-2-methyl fatty acids to S-2-methylacyl-CoA esters via an epimerization reaction prior to their degradation by β-oxidation.1 AMACR is overexpressed in localized and metastatic prostate cancer, as well as in a variety of other cancers.3 AMACR has been found selectively in patient-derived prostate cancer tumor tissue compared to non-cancer prostate tissue, and has been used as a biomarker of the disease.4 Mutations in AMACR resulting in reduced enzyme activity lead to the accumulation of R-2-methyl fatty acids and are associated with neurological and peroxisomal disorders.5 Cayman’s AMACR (N-Term) Rabbit Monoclonal Antibody (Clone RM349) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lloyd, M.D., Yevglevskis, M., Lee, G.L., et al. α-Methylacyl-CoA racemase (AMACR): Metabolic enzyme, drug metabolizer and cancer marker P504S. Prog. Lipid Res. 52(2), 220-230 (2013).

    2. Amery, L., Fransen, M., De Nys, K., et al. Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans. J. Lipid Res. 41(11), 1752-1759 (2000).

    3. Zhou, M., Chinnaiyan, A.M., Kleer, C.G., et al. Alpha-methylacyl-CoA racemase: A novel tumor marker over-expressed in several human cancers and their precursor lesions. Am. J. Surg. Pathol. 26(7), 926-931 (2002).

    4. Jiang, Z., Woda, B.A., Rock, K.L., et al. P504S: A new molecular marker for the detection of prostate carcinoma. Am. J. Surg. Pathol. 25(11), 1397-1404 (2001).

    5. Kong, G., Lee, H., Tran, Q., et al. Current knowledge on the function of α-methyl acyl-CoA racemase in human diseases. Front. Mol. Biosci. 7, 153 (2020).