For immunochemical detection of IRF4
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IRF4/MUM1 (C-Term) Rabbit Monoclonal Antibody (Clone RM352)

Item No. 32282

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Interferon Regulatory Factor 4
  • LSIRF
  • Lymphocyte-specific Interferon Regulatory Factor
  • Multiple Myeloma Oncogene 1
Immunogen
Peptide from the C-terminal region of human IRF4
Clone Designation
RM352
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) IRF4
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    Interferon regulatory factor 4 (IRF4), also known as multiple myeloma oncogene 1 (MUM1), is a transcription factor and member of the IRF family with roles in lymphocyte activation and the generation of immunoglobulin-secreting (Ig-secreting) plasma cells.1,2 It is composed of an N-terminal DNA binding domain, a linker domain, an interferon activation domain, and a C-terminal autoinhibitory region.3 IRF4 is primarily expressed in plasma cells and activated T cells but is also expressed in the heart, kidney, liver, and brain.2,3 Unlike other IRF transcription factors, IRF4 is not IFN-responsive and is activated by induction of the NF-κB pathway by various activators of lymphocyte activation and differentiation, such as LPS, concanavalin A (Item No. 14951), bacterial toxins, IL-4, and antigen receptor engagement.2 Irf4-/- mice lack Ig-secreting plasma cells, as well as exhibit defective T helper cell differentiation and decreased survival in a model of bacterial CpG-induced septic shock.4 IRF4 expression is associated with poor prognosis in patients with B cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL).5 Cayman’s IRF4/MUM1 (C-Term) Rabbit Monoclonal Antibody (Clone RM352) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shaffer, A.L., Emre, N.C.T., Lamy, L., et al. IRF4 addiction in multiple myeloma. Nature 454(7201), 226-231 (2008).

    2. Gualco, G., Weiss, L.M., and Bacchi, C.E. MUM1/IRF4: A Review. Appl. Immunohistochem. Mol. Morphol. 18(4), 301-310 (2010).

    3. Remesh, S.G., Santosh, V., and Escalante, C.R. Structural studies of IRF4 reveal a flexible autoinhibitory region and a compact linker domain. The Journal of Biological Chemisty 290(46), 27779-27790 (2015).

    4. Shaffer, A.L., Emre, N.C.T., Romesser, P.B., et al. IRF4: Immunity. Malignancy! Therapy? Clin. Cancer Res. 15(9), 2954-2961 (2009).

    5. Ito, M., Iida, S., Inagaki, H., et al. MUM1/IRF4 expression is an unfavorable prognostic factor in B-cell chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL). Jpn. J. Cancer Res. 93(6), 685-694 (2002).