For immunochemical detection of N6-methyladenosine
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

N6-Methyladenosine Rabbit Monoclonal Antibody (Clone RM362)

Item No. 32288

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • m6A
Immunogen
BSA-conjugated N6-methyladenosine
Clone Designation
RM362
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
DB, ELISA, MeRIP
Cross Reactivity
(+) m6A-RNA(+) m6A-DNA(-) Non-methylated adenosine
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    N6-Methyladenosine (m6A) is an abundant post-transcriptional RNA modification that is found in various classes of eukaryotic or viral RNA, including mRNA, rRNA, tRNA, snRNA, microRNA (miRNA), and long non-coding RNA (lncRNA).1,2 m6A modifications are enriched near stop codons of mRNA transcripts, increasing along the length of the coding region and decreasing after 3’-UTRs.1,3 m6A formation is a reversible process that occurs primarily in the nucleus.4 Its formation is catalyzed by a methyltransferase complex that is composed of the writers METTL3 and METTL14, which preferentially methylate RNA substrates with a GGACU domain, and the accessory protein Wilms tumor 1-associating protein (WTAP), which recruits target RNAs.1 m6A demethylation is mediated by the erasers fat mass and obesity-associated (FTO) protein and ALKBH5. Upon export into the cytosol, m6A modifications are recognized by a variety of reader proteins, including YTH-domain family (YTHDF) members, which regulate RNA translation. m6A modifications regulate multiple steps of RNA processing, including pre-mRNA splicing, nuclear export, translation, and decay, influencing a variety of critical cellular functions, such as differentiation, neurodevelopment, immunity, and oncogenesis.1,2,5 Global m6A mRNA levels are decreased in tumor tissue isolated from patients with endometrial cancer.6 Cayman’s N6-Methyladenosine Rabbit Monoclonal Antibody (Clone RM362) can be used for dot blot (DB), ELISA, and methylated RNA immunoprecipitation (MeRIP) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, C., Fu, J., and Zhou, Y. A review in research progress concerning m6A methylation and immunoregulation. Front Immunol. 10, 922 (2019).

    2. Yang, C., Hu, Y., Zhou, B., et al. The role of m6A modification in physiology and disease. Cell Death Dis. 11(11), 960 (2020).

    3. Meyer, K.D., Saletore, Y., Zumbo, P., et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. Cell 149(7), 1635-1646 (2012).

    4. Zaccara, S., Ries, R.J., and Jaffrey, S.R. Reading, writing and erasing mRNA methylation. Nav. Rev. Mol. Cell Biol. 20(10), 608-624 (2019).

    5. Sun, T., Wu, R., and Ming, L. The role of m6A RNA methylation in cancer. Biomed. Pharmacother. 112, 108613 (2019).

    6. Liu, J., Eckert, M.A., Harada, B.T., et al. m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat. Cell Biol. 20(9), 1074-1083 (2018).