For immunochemical detection of MMP-12
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MMP-12 (C-Term) Rabbit Monoclonal Antibody (Clone RM381)

Item No. 32305

Product Insert (PDF)
Technical Information
Synonyms
  • Macrophage Elastase
  • Matrix Metalloproteinase-12
  • MME
Immunogen
Peptide from the C-terminal region of human MMP-12
Clone Designation
RM381
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) MMP-12
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µL

    $411

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    Cayman Chemical
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    Product Description

    Matrix metalloproteinase-12 (MMP-12) is a zinc-dependent metalloproteinase and member of the MMP protein family, which has roles in extracellular matrix (ECM) degradation.1 It is composed of an N-terminal proenzyme domain, a catalytic domain that contains the zinc-binding motif, and a hemopexin-like C-terminal domain. MMP-12 is a secreted protease expressed by macrophages. It is activated by the loss of the N-terminal proenzyme domain, and this active enzyme is further processed to mature MMP-12 via loss of the C-terminal domain. Levels of MMP-12 are increased in sputum from patients with chronic obstructive pulmonary disease (COPD).2 Plasma levels of MMP-12 are increased and positively associated with atherosclerosis and adverse coronary events in patients with type 2 diabetes mellitus.3 Tumor levels of MMP-12 are positively correlated with tumor size and poor overall survival in patients with hepatocellular carcinoma.4 Cayman’s MMP-12 (C-Term) Rabbit Monoclonal Antibody (Clone RM381) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nar, H., Werle, K., Bauer, M.M., et al. Crystal structure of human macrophage elastase (MMP-12) in complex with a hydroxamic acid inhibitor. J. Mol. Biol. 312(4), 743-751 (2001).

    2. Demedts, I.K., Morel-Montero, A., Lebecque, S., et al. Elevated MMP-12 protein levels in induced sputum from patients with COPD. Thorax 1(3), 196-201 (2006).

    3. Goncalves, I., Bengtsson, E., Colhoun, H.M., et al. Elevated plasma levels of MMP-12 are associated with atherosclerotic burden and symptomatic cardiovascular disease in subjects with type 2 diabetes. Arterioscler. Thromb. Vasc. Biol. 35(7), 1723-1731 (2015).

    4. He, M.-K., Le, Y., Zhang, Y.-F., et al. Matrix metalloproteinase 12 expression is associated with tumor FOXP3+ regulatory T cell infiltration and poor prognosis in hepatocellular carcinoma. Oncol. Lett. 16(1), 475-482 (2018).