For immunochemical detection of BAG-1
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BAG-1 Rabbit Monoclonal Antibody (Clone RM356)

Item No. 32306

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • BAG Family Molecular Chaperone Regulator 1
  • Bcl-2-associated Athanogene 1
Immunogen
Peptide corresponding to human BAG-1
Clone Designation
RM356
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) BAG-1S(+) BAG-1M(+) BAG-1L
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Product Description

    Bcl-2-associated athanogene 1 (BAG-1) is a co-chaperone protein and member of the BAG family of proteins that has anti-apoptotic activity.1 It is composed of an N-terminal ubiquitin-like (UBL) domain and a conserved C-terminal BAG domain. BAG-1 has four isoforms, with the short (BAG-1S), medium (BAG-1M), long (BAG-1L) isoforms generated via alternative translation initiation sites and a BAG-1 isoform generated by post-translational modification.2 BAG-1L contains a nuclear localization signal in addition to the UBL and BAG domains and is the only isoform not found primarily in the cytoplasm.1 All BAG-1 isoforms are expressed ubiquitously and interact the anti-apoptotic protein Bcl-2 to increase its activity.2,1 Under cell stress conditions, BAG-1 binds to the ATPase domain of Hsc70 or Hsp70 with its BAG domain and inhibits DNA synthesis and cell cycling. BAG-1 interacts with additional signaling proteins, including Raf-1, which activates ERK signaling and induces cell proliferation.1 Protein levels of BAG-1 are increased in patient-derived invasive breast carcinoma tissue but, in contrast, its expression is positively associated with increased survival in early-stage breast cancer patients.3,4 Cayman’s BAG-1 Rabbit Monoclonal Antibody (Clone RM356) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the short, medium, and long isoforms of BAG-1 from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Doong, H., Vrailas, A., and Kohn, E.C. What’s in the ‘BAG’? – a functional domain analysis of the BAG-family proteins. Cancer Lett. 188(1-2), 25-32 (2002).

    2. Mariotto, E., Viola, G., Zanon, C., et al. A BAG’s life: Every connection matters in cancer. Pharmacol. Ther. 209, 107498 (2020).

    3. Tang, S.-C., Shaheta, N., Chernenko, G., et al. Expression of BAG-1 in invasive breast carcinomas. J. Clin. Oncol. 17(6), 1710-1719 (1999).

    4. Turner, B.C., Krajewski, S., Krajewski, M., et al. BAG-1: A novel biomarker predicting long-term survival in early-stage breast cancer. J. Clin. Oncol. 19(4), 992-1000 (2001).