VEGF-A (C-Term) Rabbit Monoclonal Antibody (RM391)

VEGF-A is a member of the PDGF/VEGF family of growth factors that regulates development, proliferation, and maintenance of the vascular system.¹ Alternative splicing of Vegfa pre-mRNA generates two families of variants, pro-angiogenic VEGF-A_xxx and anti-angiogenic VEGF-A_xxxb isoforms, where xxx indicates the number of amino acids in the mature protein.^2,3 VEGF-A exists as a secreted or membrane-bound homodimer composed of an N-terminal signal peptide, which is cleaved in the mature protein, a central VEGF homology domain containing the receptor-binding region, and a variable C-terminal domain containing heparin- or neuropilin-binding sites.⁴ It is secreted by numerous cell types, including endothelial cells, fibroblasts, smooth muscle cells, platelets, macrophages, and neutrophils, and is induced by inflammation or hypoxia. VEGF-A binds to VEGFR1 and VEGFR2, which are expressed by endothelial cells, to regulate angiogenesis and vascular permeability, as well as cell proliferation, survival, and migration, in an isoform-dependent manner. VEGFA SNPs have been found in patients with a variety of cancers, including breast, prostate, or colorectal cancers.² Cayman's VEGF-A (C-Term) Rabbit Monoclonal Antibody (RM391) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

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1. Shibuya, M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: A crucial target for anti- and pro-angiogenic therapies. Genes Cancer 2(12), 1097-1105 (2011).

2. Canavese, M., Ngo, D.T.M., Maddern, G.J., et al. Biology and therapeutic implications of VEGF-A splice isoforms and single-nucleotide polymorphisms in colorectal cancer. Int. J. Cancer 140(10), 2183-2191 (2017).

3. Harper, S.J., and Bates, D.O. VEGF-A splicing: The key to anti-angiogenic therapeutics? Nat. Rev. Cancer 8(11), 880-887 (2008).

4. Peach, C.J., Mignone, V.W., Arruda, M.A., et al. Molecular pharmacology of VEGF-A isoforms: Binding and signalling at VEGFR2. Int. J. Mol. Sci. 19(4), 1264 (2018).