CD14 Rabbit Monoclonal Antibody (RM415)

CD14 is a membrane glycoprotein, pattern recognition receptor, and co-receptor for several toll-like receptors (TLRs).^1,2 It is composed of a C-terminal glycosylphosphatidylinositol (GPI) anchor, several sequences associated with LPS binding, and an N-terminal hydrophobic pocket containing leucine-rich repeats. CD14 is highly expressed on myeloid lineage cells, such as monocytes, dendritic cells, microglia, and macrophages, and is commonly used as a marker of monocyte differentiation.¹ It is also expressed, to a lesser extent, in non-immune cells, and a soluble form of CD14 (sCD14) can be secreted in a protease-dependent or -independent manner to confer LPS-responsiveness to cells not expressing CD14. CD14 binds LPS in a concentration- and LPS chemotype-dependent manner to induce or suppress TLR-mediated LPS responses.^1,3 It also binds to various pathogen-associated molecular patterns (PAMPs) and heat shock protein 70 (Hsp70) to induce innate immune responses and cytokine production, respectively. CD14 deficiency inhibits LPS-induced inflammation in mouse lung, intestine, and liver and is protective against LPS- or E. coli-induced lethal inflammation in mouse models of systemic septic shock. CD14 expression is upregulated in monocytes, adipocytes, and whole adipose tissue isolated from individuals with obesity and patients with diabetes mellitus.⁴ Cayman’s CD14 (C-Term) Rabbit Monoclonal Antibody (RM415) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Wu, Z., Zhang, Z., Lei, Z., et al. CD14: Biology and role in the pathogenesis of disease. Cytokine Growth Factor Rev. 48, 24-31 (2019).

2. Schütt, C. Molecules in focus CD14. Int. J. Biochem. Cell Biol. 31, 545-549 (1998).

3. Ciesielska, A., Matyjek, M., and Kwiatkowska, K. TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling. Cell Mol. Life Sci. 78, 1233-1260 (2021).

4. Patiño, R., Ibarra, J., Rodriguez, A., et al. Circulating monocytes in patients with diabetes mellitus, arterial disease, and increased CD14 expression. Am. J. Cardiol. 85(11), 1288-1291 (2000).