For immunochemical detection of IgG
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IgG Fab (rabbit) Monoclonal Antibody - Biotinylated (RMG01)

Item No. 32358

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G
Immunogen
Rabbit IgG
Clone Designation
RMG01
MW
~150 kDa
Formulation (Request formulation change)
50 µg of protein G-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Goat
Isotype
IgG
Applications
ELISA, WB (non-reducing conditions)
Cross Reactivity
(+) IgG(-) Human IgG(-) Mouse IgG(-) Rat IgG
Species Reactivity
(+) Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $464

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1,2 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in rabbit serum. IgG consists of two identical heavy chains of approximately 50 kDa each and two identical light chains of approximately 25 kDa each.1 The heavy chains are linked together by a single disulfide bond to form an Fc region and also combine with the light chains through additional disulfide bonds to form the Fab region, which mediate receptor and antigen binding, respectively. IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.3,1,4 Rabbits express three light chains, IgGκ (K1), IgGκ (K2), and IgGγ, with IgGκ (K1)-containing IgGs comprising approximately 90% of the total IgG population. Unlike human and mouse IgG, rabbit IgG has a single subclass and a short upper and middle hinge length of only 11 residues that facilitates the hinge extension necessary for binding of the Fc receptor (FcR) and activating complement component 1q (C1q) to facilitate complement activation.2,1 Cayman's IgG (rabbit) Monoclonal Antibody - Biotinylated (RMG01) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Fab region of IgG at approximately 150 kDa from rabbit samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Allan, R., and Isliker, H. Studies on the complement-binding site of rabbit immunoglobulin G-11. The reaction of rabbit IgG and its fragments with Clq. Immunochemistry 11(5), 243-248 (1974).

    2. Rayner, L.E., Kadkhodayi-Kholghi, N., Heenan, R.K., et al. The solution structure of rabbit IgG accounts for its interactions with the Fc receptor and complement C1q and its conformational stability. J. Mol. Biol. 425(3), 506-523 (2013).

    3. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    4. Williams, R.C., Jr., Osterland, C.K., Margherita, S., et al. Studies of biologic and serologic activities of rabbit-IgG antibody depleted of carbohydrate residues. J. Immunol. 111(6), 1690-1698 (1973).