For immunochemical detection of IgG
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

IgG (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM104)

Item No. 32348

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G
Immunogen
Mouse IgG
Clone Designation
RM104
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, WB (non-reducing conditions)
Cross Reactivity
(+) IgG1(+) IgG2a(+) IgG2b(+) IgG3(+) Goat IgG(-) Mouse IgM(-) Mouse IgA(-) Mouse IgE(-) Human IgG(-) Rat IgG
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $411

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.5,6 Formulations containing humanized, chimeric, or murine IgG monoclonal antibodies have been used in the treatment of inflammatory diseases, such as ulcerative colitis, rheumatoid arthritis, and asthma, as well as cancer.7 Cayman's IgG (mouse) Rabbit Monoclonal Antibody - Biotinylated (RM104) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Fc region of IgG from mouse samples and may cross react to goat IgG.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Collins, A.M. IgG subclass co-expression brings harmony to the quartet model of murine IgG function. Immunol. Cell Biol. 94(10), 949-954 (2016).

    6. Martin, R.M., Brady, J.L., and Lew, A.M. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J. Immunol. Methods 212(2), 187-192 (1998).

    7. Lu, R.-M., Hwang, Y.-C., Liu, I.-J., et al. Development of therapeutic antibodies for the treatment of diseases. J. Biomed. Sci. 27(1), 1 (2020).