Active • Host: Insect cells • AA: 2-583 (full length) • Tag: N-terminal GST • MW: 95.5 kDa
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PARP2 (human, recombinant)

Item No. 32562

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ADPRT
  • ADPRT2
  • ADPRTL2
  • ARTD2
  • Poly (ADP-ribose) Polymerase 2
  • Poly(ADP-ribosyl)transferase 2
Purity
≥50% estimated by SDS-PAGE
Source
Active recombinant human N-terminal GST-tagged PARP2 expressed in insect cells
Amino Acids
2-583 (full length)
MW
95.5 kDa
Formulation (Request formulation change)
40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 16 mM glutathione, 20% glycerol, and 3 mM DTT
Host
Insect cells
UniProt Accession №
Q9UGN5
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $624

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    Product Description

    Poly(ADP-Ribose) polymerase 2 (PARP2) is an ADP-ribosylating enzyme with a role in the DNA damage response (DRR).1,2 It is composed of a natively disordered N-terminal region containing a nuclear localization sequence, a central Trp-Gly-Arg (WGR) domain that is essential to DNA damage-dependent catalytic domain activity, and a C-terminal catalytic domain (CAT) that contains an ADP-ribosyltransferase fold and a regulatory helical subdomain.2 PARP2 is ubiquitously expressed and localized to the nucleus where it is preferentially activated by the presence of PARP1-induced poly(ADP)-ribosylation (PARylation) induced by PARP1 (Item No. 32561) at sites of single-stranded DNA breaks. The WGR domain binds directly to DNA and activates PAR catalytic activity and branched PAR chain synthesis through cross-talk with the helical subdomain.1,2 PARP2 PARylates itself and other proteins, including transcription factors, influencing multiple biological processes, such as transcription, genome maintenance, and cell signaling.3 PARP2 expression is negatively correlated with the amount of tumor immune infiltrate in tumor samples isolated from patients with osteosarcoma.4 Cayman’s PARP2 (human, recombinant) protein can be used for enzyme assay applications. This protein consists of 582 amino acids and has a calculated molecular weight of 95.5 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chen, Q., Kassab, M.A., Dantzer, F., et al. PARP2 mediates branched poly ADP-ribosylation in response to DNA damage. Nat. Commun. 9(1), 3233 (2018).

    2. Riccio, A.A., Cingolani, G., and Pascal, J.M. PARP-2 domain requirements for DNA damage-dependent activation and localization to sites of DNA damage. Nucleic Acids Res. 44(4), 1691-1702 (2016).

    3. Ali, S.O., Khan, F.A., Galindo-Campos, M.A., et al. Understanding specific functions of PARP-2: New lessons for cancer therapy. Am. J. Cancer Res. 6(9), 1842-1863 (2016).

    4. Wu, C.-C., Beird, H.C., Livingston, J.A., et al. Immuno-genomic landscape of osteosarcoma. Nat. Commun. 11(1), 1008 (2020).