Zebrafish Retinoic Acid Receptor Alpha, isoform A Reporter Assay System

INDIGO's Zebrafish Retinoic Acid Receptor Alpha, isoform A Reporter Assay System (zfRARαa) utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the Zebrafish (Danio rerio) Retinoic Acid Receptor, Alpha A (nr1b1 isoform A), a ligand-dependent transcription factor.

INDIGO's Reporter Cells express a hybrid zebrafish RARαa receptor in which the native N-terminal ligand binding domain (LBD) has been substituted with that of the yeast GAL4 LBD sequence. Accordingly, the resident luciferase reporter gene is functionally linked to tandem copies of the Gal4 upstream activation sequence (UAS). Thus, quantifying changes in luciferase expression in the treated reporter cells provides a specific and sensitive surrogate measure of ligand-induced changes in zfRARαa activity. The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against zebrafish RARαa. In particular, zebrafish reporter assays are used in the monitoring of environmental samples for the presence of bio-active chemical pollutants.

zfRARαa Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. [INDIGO Catalog No. Z02201-32, Z02201]

WARNING This product is not for human or veterinary use.