Zebrafish Androgen Receptor Reporter Assay System

This nuclear receptor assay system utilizes proprietary mammalian cells engineered to provide constitutive, high-level expression of the Zebrafish (Danio rerio) Androgen Receptor (nr3c4), a ligand-dependent transcription factor referred to herein as zfAR.

INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to an ARresponsive promoter. Thus, quantifying changes in luciferase expression in the test sample treated reporter cells provides a sensitive surrogate measure of changes in zfAR activity. The principal application of this assay is in the screening of test samples to quantify any functional bioactivity that they may exert against zebrafish AR. In particular, zebrafish reporter assays are frequently used in the monitoring of environmental samples for the presence of biohazardous chemical pollutants, such as endocrine disruptors.

Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryopreservation method yields exceptional cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spinand-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup. [INDIGO Catalog Nos. Z03001-32 | Z03001]

WARNING This product is not for human or veterinary use.