Active • Host: Insect cells • AA: 19-417 • Tag: N-terminal His • MW: 48.3 kDa
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ROCK2 (human, recombinant)

Item No. 32758

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • KIAA0619
  • Rho-associated Coiled-coil-containing Protein Kinase 2
  • Rho-associated Protein Kinase 2
  • Rho Kinase 2
  • p164 ROCK2
Purity
≥95%
Source
Active recombinant N-terminal His-tagged ROCK2 expressed in insect cells
Amino Acids
19-417
MW
48.3 kDa
Formulation (Request formulation change)
20 mM HEPES, pH 7.4, with 150 mM sodium chloride and 1 mM DTT
UniProt Accession №
O75116
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $477

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    Product Description

    Rho-associated protein kinase 2 (ROCK2) is a serine/threonine kinase that has roles in cytoskeletal organization and autoimmunity, as well as cell survival, proliferation, and apoptosis.1,2,3 It exists as a homodimer where each monomer is composed of an N-terminal kinase domain, a coiled-coil region that contains the Rho-binding domain, and a C-terminal pleckstrin homology (PH) domain that contains a cysteine-rich C1 domain and acts as an autoinhibitory region.4,2 Rock2 mRNA is expressed in brain, muscle, heart, lung, and placenta, localized to the cytosol and nucleus, associated with the centrosome, and co-localized with actin and vimentin in a cell-type-specific manner.1,2 Binding of ROCK2 to GTP-bound RhoA induces activation of ROCK1, which phosphorylates a variety of downstream targets, including myosin light chain (MLC) phosphatase 1 (MYPT1) and MLC2, that have diverse roles in cytoskeletal organization.2 Additionally, ROCK2 is cleaved and activated by proteases, including granzyme B and caspase-2, to induce apoptosis.1 Pharmacological inhibition of ROCK2 restores immune homeostasis and shifts the helper T (Th17)/regulatory T (Treg) cell balance towards Tregs in various animal models of autoimmunity and chronic graft versus host disease (GVHD).3 Rock2 knockdown decreases the fibrogenic response in a mouse model of diabetic kidney disease.5 Cayman's ROCK2 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hartmann, S., Ridley, A.J., and Lutz, S. The function of Rho-associated kinases ROCK1 and ROCK2 in the pathogenesis of cardiovascular disease. Front. Pharmacol. 6, 276 (2015).

    2. Julian, L., and Olson, M.F. Rho-associated coiled-coil containing kinases (ROCK): Structure, regulation, and functions. Small GTPases 5, e29846 (2014).

    3. Zanin-Zhorov, A., and Blazar, B.R. ROCK2, a critical regulator of immune modulation and fibrosis has emerged as a therapeutic target in chronic graft-versus-host disease. Clin. Immunol. 230, 108823 (2021).

    4. Haga, R.B., Garg, R., Collu, F., et al. RhoBTB1 interacts with ROCKs and inhibits invasion. Biochem. J. 476(17), 2499-2514 (2019).

    5. Matoba, K., Takeda, Y., Nagai, Y., et al. The physiology, pathology, and therapeutic interventions for ROCK isoforms in diabetic kidney disease. Front. Pharmacol. 11, 585633 (2020).