Active • Host: E. coli • AA: 61-503 • Tag: C-terminal His • MW: 52.71 kDa
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Lanosterol 14-α-demethylase (human, recombinant)

Item No. 32928

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cytochrome P450 51A1
  • Cytochrome P45014DM
  • Cytochrome P450LI
  • CYP51A1
  • CYP51
  • LDM
Purity
≥60% estimated by SDS-PAGE
Source
Active recombinant human C-terminal His-tagged lanosterol 14-α-demethylase expressed in E. coli
Amino Acids
61-503
MW
52.71 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.6, with 0.5 mM EDTA, 0.2 mM DTT, and 10% glycerol
UniProt Accession №
Q16850
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $637

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    Product Description

    Lanosterol 14-α-demethylase, also known as cytochrome P450 (CYP) isoform CYP51A1, is a heme-containing demethylase and member of the CYP450 superfamily that demethylates lanosterol (Item No. 19521) to generate sterol precursors in the synthesis of cholesterol (Item Nos. 39088 | 9003100).1,2 It is ubiquitously expressed with higher levels found in the testes and ovaries and has a globular structure that is composed of a hydrophobic substrate channel, a substrate-binding cavity, and a heme-iron cofactor conjugation region.3,2 Inhibition of lanosterol 14-α-demethylase decreases cholesterol levels and induces differentiation in HL-60 promyeoloblast leukemia cells.4 Cayman's Lanosterol 14-α-demethylase (Item No. 32928) protein can be used for ELISA, enzyme activity assay, and Western blot applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Walker, K.A.M., Kertesz, D.J., Rotstein, D.M., et al. Selective inhibition of mammalian lanosterol 14α-demethylase: A possible strategy for cholesterol lowering. J. Med. Chem. 36(15), 2235-2237 (1993).

    2. Rozman, D. Lanosterol 14α-demethylase (CYP51) - a cholesterol biosynthetic enzyme involved in production of meiosis activating sterols in oocytes and testis - a minireview. Pflugers Arch 439(Suppl 1), r056-r057 (2000).

    3. Strushkevich, N., Usanov, S.A., and Park, H.-W. Structural basis of human CYP51 inhibition by antifungal azoles. J. Mol. Biol. 397(4), 1067-1078 (2010).

    4. Sánchez-Martín, C.C., Dávalos, A., Martín-Sánchez, C., et al. Cholesterol starvation induces differentiation of human leukemia HL-60 cells. Cancer Res. 67(7), 3379-3386 (2007).